Prokaryotic Expression Of Chicken Infectious Bursal Disease Virus VP2Protein

Infectious bursal disease (IBD) is caused by infectious bursal disease virus(IBDV) that infringes chicken lymphoid tissue, especially the central immune organbursa,causing huge economic losses each year in the poultry industry. The disease notonly causes the damage of tissue but also leads to immune suppression, which reducesthe body’s immune defenses and results in immunization failure. In recent years, theemergence of IBDV variants and virulent strains brings more challenge to currentvaccination strategy against IBDV. Hence, the epidemiology of IBDV infectionbecomes more complicated, posing huge obstacles for the prevention and control ofIBD. VP2protein is the major viral structural protein and the main protective antigen,Therefore, the recombinant express of VP2protein and related research can provideuseful technical support and theoretical basis for the prevention and treatment ofIBDV as well as the development of new IBDV subunit vaccine.In this paper, the reference IBDV-VP2gene was codon-optimized using JavaCodon Adaptation Tool (JCAT) software, synthesized, and cloned into pUC-57vector.Upon double enzyme digestion of pUC57-VP2and pET28a, recombinant plasmidpET28a-VP2was constructed by ligating the enzymatic products. Recombinantplasmid pET28a-VP2was transformed in BL21(DE3) Plyss.With IPTG inductionexpressing,SDS-PAGE analysis showed that the protein appeared in48ku, whichwas mainly soluble and was consistent with the oretical molecular weight of theprotein. Recombinant protein pET28a-VP2accounted for10%of the total proteinexpressed by E. coli. Western blot showed that VP2protein can be recognized byanti-His mAb. The preparation of VP2protein in this study forms the first step fordeveloping IBDV subunit vaccine...