Bioinformatics Analysis Of Short-chain Dehydrogenase/Reductase Family SDR110C And Genetic Transformation Of CsSDR110C14in Cucumber

Cucumber (Cucumis sativus L.) is an important facility cultivation vegetable in China. Fruit warty trait is one of the highly valuable external quality traits of cucumber. Cucumber fruit spines and trichomes have common characteristics and genetic basis, so study on cucumber trichomes helps people understanding the developmental mechanism of cucumber fruit warty. Glabrous Cucumber(glabrousl, gll) was a nature mutant found in North China type cucumber varieties (’Daqingba’). In order to study gene regulatory network of the trichome formation key genes Gll, we identified the differential expression genes between gll and ’Daqingba’. In the differential expression genes, CsalM168880was one of the up-regulated genes in glabrousl (gll) mutant. The bioinformatics datas showed that CsalM168880belonged to a member of the short-chain dehydrogenase/reductases SDR110C family. Bioinformatics method predicted function of CsalM168880and its homologous genes. The tissue expression patterns of these genes were examined in various cucumber organs. According to the SDR nomenclature initiative, the gene was named CsSDR110C14. We used the reverse genetics methods to study CsSDR110C14function in the growth and development of cucumber and screened regulation transcription factors of CsSDRl10C14. To determine whether CsSDR110C14involved in the formation of cucumber trichomes. Our main results are as follows:1. The homologous genes of CsSDR110C14were searched and a total of18homologous genes were identified. The phylogenetic relationship analysis showed that these18CsSDR110C genes participated in the different molecular biology process.2. Tissue specific expression showed that cucumber18SDR110C genes expression patterns were different in roots, stems, leaves, male flowers, fruits and tendrils. Part of this genes expression level increased in the light-induced cucumber seedlings detiolation process.3. Overexpression and silencing vectors of CsSDR110C14were constructed and genetic transformation, got2overexpression CsSDR110C14regenerated plants growth was unnormal This phenotype also needed further identification by getting genetic stability transgenic lines of this gene.4. A bait vector containing promoter of CsSDR110C14was constructed and20candidate proteins may regulate the expression of CsSDR110C14. Csa3M826680was one ZF-HD transcription factor of zinc finger HOMEOBOX proteins family, may regulate the expression of CsSDR110C14.In summary, using a bioinformatics approach, short-chain dehydrogenase/reductase CsalM168880and its homologous genes function were predict and preliminary studied the function of Csal Ml68880gene by using reverse genetics approaches. This laid the foundation for further elucidating the biological function of CsalM168880gene.