Rna Editing Analysis Of Mitochondrial Gene In Cytoplasmic Male Sterile Line And Maintainer Line In Soybean

Cytoplasmic male sterility is the basis for crop utilization of heterosis, research the causes of cytoplasmic male sterility is not only of great theoretical significance, but also has important production value in use. Soybean is the world’s important crops, oil and chemical raw materials compared with other crops, soybean cytoplasmic male sterility research started late, but develop rapidly. In order to make better use of cytoplasmic male sterile line of soybean that can be used to produce hybrids, study the molecular mechanism of soybean cytoplasmic male sterility is great significance. Numerous studies show that RNA editing of mitochondrial genes and cytoplasmic male sterility have a variety of contact. RNA editing can cause mutations in mitochondrial genes including nucleotide insertion, deletion or substitution, which affects the splicing of the primary transcripts, resulting in cytoplasmic male sterility.RN-type cytoplasmic male sterile line and its maintainer line JLCMS9B JLCMS9A and ZD-type cytoplasmic male sterile line and its maintainer line JLCMSZ9B JLCMSZ9A which were bred by Jilin Academy of Agricultural Sciences were used in this study. Comparative analysis the relationship between RNA editing sites of soybean mitochondrial function and energy metabolism genes atpl, atp4, atp6, atp9, cox Ⅰ, cox Ⅱ, cox Ⅲ and cob and RN-type cytoplasmic male sterility. The results showed that not RNA editing occurs in atp4and cox II between JLCMS9A and JLCMS9B; RNA editing occurs in atp9,cox Ⅰ, cox Ⅲ and cob gene does not affect the change encoded amino acid; However, atp6gene has three more editing sites in JLCMS9A than JLCMS9B, two of which caused changes in amino acid and prediction of protein structure. These results suggested that this change is most likely to cause the abnormal function of atp6gene in JLCMS9A and lead to cytoplasmic male sterility. Compare with JLCMSZ9A and JLCMSZ9B, no RNA editing that has happened in atp6, cox Ⅱ and cob; RNA editing occurs in atpl, atp4, atp9, cox Ⅰ and cox Ⅲ does not affect encoding amino acid change. Therefore RNA editing that may not relationship with ZD-type cytoplasmic male sterility.