Analysis Of Nitrifying Bacteria And Denitrifying Bacteria Diversity In The Composting Process

Composting is to rely on microbe widely distributed, in artificial conditions, effectively control can promote the biochemical process into humus biological degradation of organic compounds and stable, and its essence is a kind of fermentation process. Composting is harmless, reduction of solid waste as well as the fundamental way of resources. Study on composting of organic solid wastes has been one of the hot research field of environmental science. Study on analysis of nitrifying bacteria and denitrifying bacteria in composting dynamics and diversity plays a decisive role for the effective control of the composting process.The experiment is based on amoA gene and nosZ gene as the research object, study on composting of solid fermentation process of nitrifying bacteria and denitrifying community structure and dynamic changes of the PCR-DGGE technology, to clear the composting process of nitrifying bacteria and denitrifying bacteria community and its changes in different stages, so as to improve the efficiency to provide more theoretical basis for the rich and accurate movement reveals part of nitrogen in compost and reduce nitrogen loss.The main results are as follows:through the analysis of DGGE spectra, also from the natural compost or compost samples diversity of nitrifying bacteria to analysis, its diversity Jun showed a downward trend after the first rise. Compost samples nitrifying bacterial diversity than natural compost, contrast agents to process denitrifying bacteria diversity in the sample is lower than the natural compost. In the DGGE spectrum bands were gel extraction, cloning, sequencing, and compared with known sequences in GeneBank that includes the nitrifying bacteria compost in the5groups,respectively,β-proteobacteria,y-proteobacteria,Bacilli,Planctomycetes and Actinobacteria. The β-proteobacteria accounted for all of the detected strains was44.4%, nitrifying bacteria groups mainly compost environment; denitrifying bacteria consists of four groups, respectively, a-proteobacteria,β-proteobacteria,γ-proteobacteria and8-proteobacteria. The γ-proteobacteria accounted for all of the detected strains was55.6%, denitrifying bacteria were dominant in composting.