| The ginger is the perennial grass of ginger zingiberaceae as also named ancient ginger. Ginger is not only traditional flavor vegetables, but also a kind of important economic crops. Due to long-term planting, harmful pathogenic microorganisms steady accumulation and soil disease became serious and cased ginger yield and quality dropped significantly.Depand on14kinds of pathogenic bacteria for target bacteria of Alternaria alternate, Phythium myriotylum, Ralstonia solanacearum. Measured its antibacterial spectrum through tablet confrontation of5strains. GJ3and NS178have antagonism for more than ten kinds of pathogenic bacteria. R2/R1between1.0and3.0, Have wide antimicrobial spectrum, better antagonism ability. GJ130and NS87has good antagonism effect on bacterial wilt fungus and tomato withered leaves fungus. NS111have not antagonism effect with High yield and indole acetic acid.After morphological observation, physiological and biochemical characteristics and16S rDNA sequence analysis. GJ3is Bacillus amyloliquefaciens, GJ130is Streptomyces nashvillensis, NS87is Leclercia adecarboxylate, NS111is Enterobacter ludwigii and NS178is Bacillus subtilis. The registration number of16Sr DNA sequence in GenBank and respectived:KC312154, KF958192, KC312155, KC312156and KC312157.In order to research the promoting results of antibiological inoculant. We filled field trials in Zhaojiaquan villages Laiwu city Shandong province, and set up5processing of random permutation with each processing three repetition of GJ3, GJ130NS178mixture bacteria and water contrast. The time of spray bacteria is19th April and3th August. Determination of stem circumference, plant height, branch number, the floor weight, ginger weight and so on. The results is GJ3can significantly increase the number of branches and yield, grew better than the control group. It is remarkable that by using NS178bactericide, the output per mu increased by108%. We had gathered5managed soli samples in the harvesting time. Using T-RFLP and statistial method of PCA and CCA to explore the microbial community structure diversity of soil. After analysis of T-RFLP atals, giger community structure in the rhizosphere bacteria diversity present significant differences compared with control. Input the T-RFs data to Phylogenetic the Assignment Tool database and marched. Seculate the advantages species of peak may represent. The result manifest of represent species of atlas peak in advantage is Halobacteroides elegans, Chrysiogenes arsenatis, Rhodospirillum sodomense, Streptomyces rimosus. Diversity index statistics in Bio-Dap software of6samples. Compute with biological agent, the bacteria community structure is plentiful of control group. The bacterial population structure similarity show obvious difference among samples. The biological bacterium agent of microbial community structure tended to be simple, lower diversity, resulting in a decline in resistance of undispose.In order to obtained high concentraions of bacteria. This topica also optimization the culture medium of single factor test optimizing the bacteria count and the rate of spore conditionson the basis of the bean sprout juice medium of NS178, and got three related factors of glucose,(NH4)2SO4and K2HPO4. The Box-Behnken Design method be used to optimized the fermentation medium. By the analysis of optimum conditions for the response surface method is2.29%glucose,0.32%(NH4)2SO4and0.23%K2HPO4. And optimization the culture conditions such as temperature, pH, rotational speed, liquid loading quantity respectively is33℃, pH7.5,180rpmrpm-min-1,50mL. Under the optimal culture conditions, NS178biomass increased to34.50×108cfu-mL"1, cultiv spore rate was75.8%after72h, the fermented liquid antagonism effect is enhanced.This study for bio-bacterial manure provides the reliable theory basis by used optimize the soil environment and relieve the happening of the disease, improve the yield, laid a solid foundation for the development and application of bacterial species resources. |
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