Isolation And Whole Genome Sequence Analysis Of Avian-Like H1N1Influenza Virus In Guang Xi

Virologic and serological studies were conducted on swine nasal mucus and serum samples collected from Guiping in Guangxi. The results indicated that three of365samples, were positive for influenza virus, the isolate rate was0.82%. All the three isolates were determined as H1N1subtype influenza virus by hemagglutination-inhibiton and whole genome sequencing, which named G14、G21、G30, respectively.Sequence analysis confirmed that G14、G21、G30isolates were high related with A/swine/Zhejiang/1/2007, and the amino acid receptors were108(Y)、148-152(GVTAA)、148(W)、197(H)、204(D)/208(L).209(Y),respectively,which was the same with A/swine/Hungary/19774/2006and A/swine/Zhejiang/1/2007. Analysis of glycosylation sites found that HA1had5glycosylation sites, HA2had2glycosylation sites,which were27(NNST)、28(NSTD)、40(NVTV)、212(NHTY)、291(NCTT)、498(NGTY)、557(NGSL), respectively. G14、G221、G30and A/swine/Zhejiang/1/2007had the same glycosylation sites, but21increased to A/swine/Hungary/19774/2006. Increase or decrease of glycosylation sites lead to the change of pathogenicity、antigenic characteristics and chemoresistance. Analysis NA of glycosylation sites found that NA had6glycosylation sites,that were235(NGSC)、146(NGTI)、88(NSSL)、68(NVSN)、63(NQTY)5、8(NNTW), which was the same with A/swine/Zhejiang/1/2007.but G21of68glycosylation site (NVSN), difference with A/swine/Zhejiang/1/200768glycosylation site (NISV). These date suggested that G14、G21、G30probably changed in pathogenicity、antigenic characteristics and chemoresistance。 M genes play a large role in drug resistance of the virus,,according to the drug tests conford that L26F、V27A、A30T、S13N、G34E one of these variations may occur directly affect the resistance of the virus。 The three virus strains isolated from experimental variation of these sites not change that means that three strains of the virus Not resistant to amantadine drugs.