Whole Genome Resequencing Research Of Pseudomonas Aeruginose Of The Horse

Pseudomonas aeruginosa (’PA’ hereafter) is a ubiquitous environmental bacterium that is an opportunistic human and other animal pathogen. PA infections are common in patients with compromised antibacterial defenses and PA has the ability of acquired drug-resistance by inducing antimicrobial. This study involved with construction of TruSeq DNA Library, whole genome resequencing by Illumina Miseq techinique, screening SNPs and InDels, and functional annotation for PA isolated from the blood of the horse with wound infection, which lay the foundation for further study on the pathogenic mechanism of PA in horses. Results are as follows:1whole genome resequencingWe obtained about510,000pairs of reads which of length are170-220bp and inserted size is450bp. The matching rate and coverage between obtained sequencing data and reference genome are93%and96.8%respectively. The total number of7990SNPs were identified including7903homozygous SNPs and87heterozygous SNPs, at the same time total99InDels were identified including97homozygous InDels and2heterozygous InDels.2functional annotationCOG and GO functional annotation based on the distribution of SNPs and InDels was mainly classified as amino acid transport and metabolism, signal transduction, secondary metabolite biosynthesis, transport and catabolism, membrane synthesis, ATP binding, redox processes, DNA template and transcriptional regulation, phosphorelay signal transduction system and some other unknown functions. The KEGG pathway analysis of the distribution of SNPs and InDels revealed metabolic pathway and two component system.