Research Of Genes Expression May Associate With Bovine Follicular Development

In bovine, usually one pup can be generated once, and only one ovum was released from a mature follicle, the other follicles were destined to undergo apoptosis and can’t ovulate, in one estrous cycle, and restricted the rapid development of the cattle industry.Oocyte was growing, developing with the regulation of endocrine hormone, to which the genes related were expressed accurately at different stages and space. Some of the possible molecules (gonadotropin, growth factors, c-kit) has been studied, but the mechanism of inhibition of follicle growth and development was few known. In this study, follicles were collected from bovine, deep sequencing technology was performed to filter out10genes associate with bovine follicular development. QRT-PCR technology was performed to further research the10genes at a different stage, designed to explore the reproductive performance of bovine, and provide a theoretical basis for the regulation of bovine reproduction. Our fingings and conclusions were as follows:1. Studies reported the transcriptome analysis of bovine follicle at predeviation.43,708,132and44,082,301clean reads were sequenced from two libraries constructed from ODF1and PDF1, respectively. Between the two libraries,83differentially expressed genes were detected,42were up-regulated genes, and41were down-regulated genes.10putative genes were selected from the database might related to follicle development.2.Real-time PCR result indicates that MAPK13and CPXM1mRNA amounts in subordinate follicle granulose cells were extremely significantly higher than that in dominant follicle granulose cells(P<0.01). And ARID4B mRNA amount in subordinate follicle granulose cells was significantly higher than that in dominant follicle granulose cells(P<0.05). The expression levels of PRSS23and CYP19A1in dominant follicle granulose cells were extremely significantly higher than that in subordinate follicle granulose cells(P<0.01). The expression levels of PRSS35and NOTCH1in dominant follicle granulose cells were significantly higher than that in subordinate follicle granulose cells(P<0.05). But the mRNA amounts of PPP2R2A, SPOCK2and PTGFR have no significant difference between the two types of granulose cells.3. MAPK13, CPXM1and ARID4B can inhibit the bovine follicular development, while PRSS23, CYP19A1, PRSS35and NOTCH1can promote the bovine follicular development.