Effect Of Coupling Between Water And Nitrogen On Zhengdan958’s Nitrogen Metabolism, Yield, Quality And Its Related Physiological Characteristics

The experiment studied the effect of water-nitrogen coupling onZhengdan958under the condition of the field between2012and2013.The researchincludes agronomic traits, accumulation of dry matter, nitrogen metabolism,photosynthesis characteristic, ear leaf physiological characteristics, production andgrain quality. The main results were as follows:1The effect of different water and nitrogen coupling on agronomic traitsPlant height mushrooms in earlier stage and basically remain unchanged in latestage.Dynamic changes of stem diameter and leaf area shows a monopeak curvewhole developing stage. Stem diameter reaches maximum in the maleterad stage, andplant height and leaf area reach maximum value in spinning stage. Under the samenitrogen treatment, the indicators above of the W1are lowest among water treatments.Under the same water treatment, the indicators raise at first and lower later with theincreasing of nitrogen fertilizer applied in.The reason is that the double factor (waterand nitrogen)interactions. W2N2, W3N2agronomic traits are best.2The effect of different water and nitrogen coupling on accumulation of dry matterThe total dry matter accumulation in an s-shaped type.From anthesis stage tofilling stage dry matter accumulates fastest. Under the same water treatment, theamount of dry matter under the condition of N0is lowest and much lower than othernitrogen treatments. Under W1, dry matter accumulation achieves the maximumlevel in N1, while it tend to reach maximum at the N2under W2.W3has the same lawof W2. And under the same nitrogen level, the pattern of dry matter accumulation isW3> W2> W1. There is the no obvious difference between the W3and W2,whileboth of them are far greater than W1. Dry matter accumulation of Different organs indifferent stages comply with the law.3The effect of different water and nitrogen coupling on nitrogen metabolism In anthesis stage, nitrogen content of leaf is the highest, and in the mature period,most of nitrogen storage in grain, which has the highest nitrogen ratio.Along with thegrowth period, nitrogen transform from vegetative organs to reproductive organs.Under the same water treatment, the nitrogen content between N2and N2is nearlythe same, but both have significant differences with N0treatment.Under N0treatment,the nitrogen content of W1, W2, W3in different organs is not very different, yetunder the N1, N2and N3treatment, there are differences in the nitrogen content ofdifferent organs, which shows that the nitrogen interact water. W2N2nitrogen contentin grain and nitrogen ratio is the highest. N harvest index and nitrogen partialproductivity also largely in line with the law.4The effect of different water and nitrogen coupling on photosynthesis characteristicLater in flowing period, stomatal conductance, intercellular CO2concentration,net photosynthetic rate and ear leaf chlorophyll content is continuous falling.Especially in the level of W1, the above indexes reach minimum and decline fastest.Increased nitrogen relieves the declining rate of the index, the indicators of W2N2arethe most ideal.5The effect of different water and nitrogen coupling on ear leaf physiologicalcharacteristics5.1The effect of different water and nitrogen coupling on active oxygen metabolismand membrane lipid peroxidationAlong with the growth period, SOD, CAT and POD activity are falling, and thecontent of MDA is constantly rising. For the same water treatment, increasingnitrogen not only helps to raise the activity of SOD, CAT and POD,but also slows theoxygen removal rate of enzyme activity decline. For the same nitrogen treatment, weget the rule:W3> W2> W1, of which the former two don’t have big difference butmuch higher than the W1.The change of MDA content is on the contrary of theenzyme activity changes. With the increasing of nitrogen fertilizer, MDA contentreduce constantly.W2N2, W3N2have the highest enzyme activity and lowest MDAcontent.5.2The effect of different water and nitrogen coupling on carbon and nitrogenmetabolism enzyme activity in ear leafUnder the same water treatment,the GS, SPS activity of the same nitrogentreatment are higher than the N0.And under the same nitrogen treatment, W3does notdiffer from W2very much, but W3、W2are much higher than W1.Under the same water treatment, GS,SPS activity under N2nitrogen treatment is the largest. Under thesame nitrogen treatment, GS, SPS activity present the rule that W3>W2>W1,and W1is far less than the former two.W2N2, W3N2have the highest activity of twoenzymes.5.3The effect of different water and nitrogen coupling on Endogenous hormones inear leafFor W1, ABA content is greater than W2, W3and ABA content in the latesurge.Nitrogen fertilizer can reduce ABA content,but in late growth period the effectof increaseing ABA is very limited. W2, W3, processing of ABA content is lessthan W1and the increase of ABA content in the reproductive period is more uniformand slower. The ABA content of W2N2is lowest.The dynamic changes of GAcontent in corn ear leaf are on the contrary of ABA content. With the increasing ofnitrogen fertilizer, the GA content under the same water treatment i reduces afterincreasing firstly.And basically it reached the maximum in N2. Each nitrogentreatment is significantly higher than N0. For the same nitrogen treatment, the GAcontent assumes W3> W2> W1.The difference between W2and W3is not obviousbut much higher than W1. W2N2, W3N2and W3N3treatment of GA content is thehighest.6different water and nitrogen coupling effects on the yield and quality of maizeFor W1, production reaches the maximum in N1.For W2, the nitrogen treatmentis significantly higher than N0, and the production has reached the highest in N2.Afterthat, increase the nitrogen input will not result in an increase in output.W3shows thesimilar processing like W2. For N0under different water treatments,the productionincreases with the increaseing of water. Throughout the water and nitrogen treatment,the maximum yield reached in W2N2treatment.Under the condition of water stress(W1), total starch content and crude fatcontent is significantly reduced, and increased with the increasing of nitrogenfertilizer. Under the same nitrogen level, total starch content and crude fat content ingeneral for W3> W2> W1, while W2and W3content is markedly higher than W1.Changes in concentrations of amylopectin and total starch content, and amylosecontent on the other hand. Protein content present that W1W2> W3under the samenitrogen treatment. Under the same water treatment, with the increasing of nitrogenfertilizer applied protein content increase. Each index of W2N2is higher than others.