The Effect Of Jujuboside On Expression Of Genes In Relation To Feed Intake Control In Heat Stress AA Broiler Hypothalamus

ObjectivesWe study the five intake adjusting factor of heat stress AA broilerchickens hypothalamus and the relationship among the interrelationbetween the feed intake and regulation genes, and to determinejujuboside to improve heat stress ratewas feed intake of thehypothalamus mechanism and provide theoretical basis for heat stressregulation of chicken.We research that NPY, MC4R, GABAA-α1,POMC, AgRP on thehypothalamus expression which it is under the condition of normaltemperature raising and hot chicken feed processing under stresshypothalamic, exploring jujuboside that it improve heat stress ratewasfeed intake of the hypothalamus mechanism, for the rational utilizationof jujuboside chicken heat stress resistance to lay the theoreticalfoundation, rich chicken feed intake regulation theory.MethodsWe achieve experimental samples through feeding experiment.Feeding experiment adopt completely random design. At4weeks wedesign the control group and adding1.0mg/kg jujuboside group. At6weeks we design normal control group and heat stress treatmentgroup at34℃. Heat stress treatment components heat stress control,heat stress before adding jujuboside group, adding acid jujubosidegroup during heat stress and heat stress before and after adding acidjujuboside group. The AA chicken hatchery from goods has beenbougtht when they are at1age and identification of male and female. According to the average weight of breeding to2weeks, weight is toobig or too small chicken, male and female chicks were randomly375,random points5groups, each component3repeat, press bar weighing.28days of age, each repeated random take5feather, slaughteringsampling. Age of29, heat stress within20min will handle thehenhouse temperature up to34℃, and maintain in34℃±1℃. Everyweekend metering per column feed intake and body weight.43days ofage, each repeat randomly selected5, only used for samplingslaughter.We compound peptides of AgRP, GABAA-α1, MC4R, and POMC toprepare the polyclonal antibody. Adopt the method ofimmunohistochemical to check the expression of chicken NPY, AgRP,GABAA-α1, MC4R and POMC on the hypothalamic by using IPP6.0image analysis software and SPSS software to analyze data results.Results4weeks of poultry fodder add zizyphus jujube RenZao glycosides,the hypothalamic NPY, MC4R, GABAA-α1, POMC had no significanteffect (P>0.05); significantly reduced the rooster hypothalamus AgRPexpression quantity (P<0.05).6weeks heat stress treatment, brought to the hen hypothalamicNPY expression had no significant effect (P>0.05), the hypothalamusMC4R, male hen GABAA-α1, increased the rooster head issueinggrave POMC expression difference was not significant(P>0.05); Henthe hypothalamus POMC expression, the cock the hypothalamusAgRP expression significantly lower (P<0.01).The heat stress of poultry fodder add jujuboside, the treatmentgroup of hypothalamic NPY expression difference was not significant(P>0.05). Compared with the thermal stress in the control group, thecock fodder add jujuboside during heat stress jujuboside, significantlyreduced the hypothalamus MC4R expression (P<0.05), the hen beforeduring the thermal stress, thermal stress and thermal stress during the fodder add jujuboside, significantly reduced the hypothalamus MC4Rexpression (P<0.01). Compared with heat stress hen controls, thermalstress and thermal stress during the fodder before adding jujubosides,significantly improve the hen the hypothalamus GABAA-α1expression(P<0.01). Compared with heat stress the cock, the thermalstress during the fodder add jujuboside, significantly improved therooster hypothalamus POMC expression (P<0.05); Compared withheat stress hen controls, thermal stress, thermal stress during the addfoods before jujuboside, respectively, significantly improvedsignificantly and the hypothalamus POMC hen expression (P<0.01,P<0.05). Compared with heat stress the cock controls, thermal stressand thermal stress during the fodder before adding jujuboside,significantly improve the rooster hypothalamus AgRPexpression(P<0.01); Compared with the hen heat stress control,fodder add jujuboside group were significantly or extremelysignificantly improved the hen the hypothalamus AgRP expression.Multi-factor analysis of variance showed that the gender significantlyaffect6weeks broilers hypothalamus MC4R expression(P<0.01);Fodder add jujuboside, significantly affect the chicken head issueinggrave POMC, MC4R, AgRP expression(P<0.01); Interaction, genderand temperature significantly affected the hypothalamus AgRPexpression(P<0.05), significantly affect the hypothalamus POMCexpression(P<0.05); Gender and jujuboside interactions, significantlyaffect the hypothalamus POMC expression(P<0.05), significantlyaffect the hypothalamus MC4R expression(P<0.05).Heat stress treatment and fodder add jujuboside, changed thehypothalamus intake the interrelation between the amount of relatedgene expression. Correlation analysis showed that the POMC isnegatively related to the AgRP(r=-0.962, P<0.05), NPY and AgRPnegative correlation (r=-0.715—-0.970, P<0.05), MC4R and AgRPpositive correlation (r=0.990-0.999, P<0.05), POMC and NPY positivecorrelation (r=0.999, P<0.05), and POMC and MC4R negative correlation (r=-0.744—-0.999, P<0.05), POMC is negatively related tothe GABAA-α1(r=-0.965, P<0.05), NPY and MC4R negativecorrelation(r=-0.985—-1.000, P<0.05),GABAA-α1positively related tothe MC4R (r=-0.951, P<0.05).Poultry feed intake and the hypothalamus MC4R express negativecorrelation (r=0.568, P<0.05). Expressed in the hypothalamus MC4Rbetween feed intake and the mathematical model for e^(4.657294+0.025591x)(g/d per bird).ConclusionsThis experiment through synthetic peptides, immune the NewZealand white rabbit, the serum separation and purification, thesuccessful preparation of the MC4R, GABAA-α1, POMC, AgRP peptideantibodies.The thermal stress on the chicken hypothalamic NPY, GABAA-α1have no obvious effect on the expression of quantity, and significantlyimprove the MC4R hen the hypothalamus, the cock and the hen POMC,significantly reduced the amount of AgRP expression of hypothalamusrooster. MC4R, POMC, AgRP may be chicken feed intake of genesdirectly.4weeks in the normal temperature of poultry fodder addjujuboside, the expression of hypothalamus, MC4R, POMC NPY hadno significant effect, significantly improve the hypothalamus henGABAA-α1, significantly reduced the rooster hypothalamus AgRPexpression, may be the mechanism of action of acid jujubosideincrease feed intake.6weeks of thermal stress of poultry fodder add jujuboside,significantly reduced and the hypothalamus MC4R, raise the hen henGABAA-α1, hen POMC, male hen AgRP expression; Gender jujubosidein the hypothalamus POMC, MC4R expression quantity of a significantinteraction relationship, prompt jujuboside may be through regulatingchicken MC4R hypothalamus, GABAA-α1, POMC, AgRP express quantity have improved heat stress state poultry productionperformance; There are differences in approaches to the readjustmentof the hen.Poultry feed intake and the hypothalamus MC4R expression issignificantly negative correlation, the relevant mathematical model isset up.