The Effects Of Methy1Jasmonate And Salicylic Acid On Inulin Content Of Jerusalem Artichoke Suspension Cells

Jerusalem artichoke (Helianthus tuberosus L.) is a perennial herbaceous plant, belongs to the genus of Helianthus L., it has the strong resistance, it can grow in the bad environment such as drought, salt and alkali, cold, etc, has been extensively cultivated at home and abroad. There is a large amount of inulin in the Jerusalem artichoke tubers, inulin is a polyfructose that has variety physiological functions, it is widely applied to the pharmaceutical and food industries now.It is a new method to produce some secondary metabolites by using cell suspension culture to enhance the contents of plant secondary metabolites. In this text, choose the Jerusalem artichoke tubers as materials, induced the tubers callus, and established the suspension culture of Jerusalem artichoke tubers, and optimized the condition of suspension culture. Used the methyl jasmonate and salicylic acid as elicitor, research the effects on inulin content of Jerusalem artichoke suspension cells, it supplied the industrialization theory for using cell suspension culture to produce inulin.The results showed as below:(1) Firstly the research induced the Jerusalem artichoke tubers callus, obtained the equal, yellow-white color callus in MS medium with NAA1.0mg/L+6-BA1.0mg/L.(2) Established the suspension culture of Jerusalem artichoke tubers used the callus transferred to liquid MS medium with NAA1.0mg/L+6-BA1.0mg/L. Defined the suitable condition was pH5.8-6.0+30g/L sucrose+0.5mg/L praline+lmg/L glutamine. The cells grew best in the condition. Established the growth curve of the suspension culture and the changing curve of pH, the cells entered the logarithmic phase at the6th day, the increment was maximum at the18th day.(3) Established the method to determine the inulin content used the HPLC (High Performance Liquid Chromatography). Used the ELSD, the moving phase were acetonitrile and water (acetonitrile:water=78:22), the flow rate was1mL/min, the column temperature was30℃, the injection time was20min.(4) Defined the suitable concentration of MeJA was100μmmol/L through adding the different concentration of MeJA to the suspension culture, the biomass was maximum, was7.908mg/mL, the inulin content was maximum, was21.3%, the inulin yield increased71.13%compared with the control group. Defined the suitable time of MeJA was6th day through adding the MeJA to the suspension culture at different days, the biomass was maximum, was7.888mg/mL, the inulin content was maximum, was21.5%, the inulin yield increased72.35%compared with the control group. (5) Defined the suitable concentration of SA was60μmmol/L through adding the different concentration of SA to the suspension culture, the biomass was maximum, was7.73mg/mL, the inulin content was maximum, was17.3%, the inulin yield increased71.13%compared with the control group. Defined the suitable time of SA was6th day through adding the SA to the suspension culture at different days, the biomass was maximum, was7.724mg/mL, the inulin content was maximum, was17.42%, the inulin yield increased36.74%compared with the control group.