| The ascites syndrome(AS) of broiler, which is also called pulmonary hypertension syndrome(PHS) in broiler. A variety of pathogenic factors together lead to AS. The main pathological features:yellow ascites accumulation in the abdominal cavity, right ventricular hypertrophy, significant pathological damage in the heart, lungs, kidneys and other organs. AS was conducted various studies by domestic and foreign scholars. Currently more widely accepted pathogenesis of AS is that pulmonary hypertension hypothesis, the key aspect is the pulmonary hypertension which caused by hypoxia. Pulmonary hypertension with the dysfunction of vascular endothelial cells secrete factors is closely related. Hypoxia conditions cause the body relative hypoxia, a lot of oxgen free radicals were generated, oxidative stress and lipid peroxidation was occurring, vascular endothelial cells be damaged. The injured vascular endothelial cells lead to overexpression of hypoxia-inducible factor-1(HIF-1), the downstream gene of HIF-1, vascular endothelial growth factor (VEGF) corresponding overexpression. At the same time, a large number of red blood cells freshmen and extent of blood viscosity increased. This has led to blood through the lungs get more resistance, pulmonary hypertension generated. In recent years, the study of Chinese medicine to prevent AS attracted widespread attention and achieved good results. Our previous clinical trials showed Qi Ling Gui Fu Fang has a preventive effect on AS.This study selected AA broilers, using the multi-factor method induced ascites syndrome pathological model. Experimental animals were divided into six groups:control group, model group, Qi Ling Gui Fu Fang high-dose group (2g/kg·d), middle-dose group (1g/kg·d), low-dose group (0.5g/kg·d) and VC group (0.9%). Through analyzing the weight, the blood biochemical indicators(automatic blood analyzer), the T-SOD activity(xanthine oxidase method), the content of MDA(thiobarbituric acid method), the expression levels of HIF-1a and VEGF in lung and vascular tissue(real-time fluorescent quantitative method) in different groups, as well as to study on the control effect and mechanism of Qi Ling Gui Fu Fang(QLGFF) on AS. The main contents of the test and results are as follows:1. Select AA broilers, using low-temperature, high-fat, high-energy and high-sodium method(breed at9～11℃,water add0.12%Na+, fodder add3%lard and4%fish meal), AS pathological model was constructed successfully.2. Compared with the control group, the model group body weight was significantly decreased(P<0.05). Compared with the model group, the high-dose of Qi Ling Gui Fu Fang group and VC group body weight was significantly increased(P<0.05).3. Compared with the control group, model group blood indices:MCH, MCHC, TP, ALB, GLB, A/G was significantly decreased (P<0.05), WBC, RBC, Hb, PCV, PLT, MPV and PCT were significantly increased (P<0.05). High-dose of Qi Ling Gui Fu Fang group significantly improved each blood indices (P<0.05). Middle-dose group, low-dose group and VC group could improve different ages of AS some blood indices(P<0.05).4. Compared with the control group, the model group T-SOD activity was significantly decreased (P<0.05). Compared with the model group, high-dose of Qi Ling Gui Fu Fang group on35,45,55days of age T-SOD activity significantly increased (P<0.05), and the middle-dose of Qi Ling Gui Fu Fang and VC group on45,55days of age T-SOD activity were significantly increased (P<0.05).5. Compared with the control group, MDA content in model group increased significantly (P<0.05); Compared with the model group, MDA content in the high-dose of Qi Ling Gui Fu Fang group was significantly decreased(P<0.05), VC group MDA content on35,45,55days of age were significantly decreased(P<0.05), the middle-dose of Qi Ling Gui Fu Fang group MDA content on45,55days of age were significantly decreased(P<0.05), the low-dose of Qi Ling Gui Fu Fang group MDA content on55-day-old was significantly decreased(P<0.05).6. Compared with the control group, the amount of VEGF gene expression in lung tissue of model group was significantly increased(P<0.05); Compared with the model group, high-dose and VC group in the lung tissue VEGF gene expression were significantly decreased(P<0.05), the middle-dose and low-dose group had no significant difference(P>0.05).7. Compared with the control group, the amount of VEGF gene expression in vascular tissue of model group was significantly increased(P<0.05); Compared with the model group, high-dose, middle-dose, low-dose and VC group in the vascular tissue VEGF gene expression were significantly decreased(P<0.05).8. Compared with the control group, the amount of HIF-1α gene expression in lung tissue of model group was significantly increased(P<0.05); Compared with the model group, high-dose group and VC group in the lung tissue HIF-la gene expression were significantly decreased(P<0.05), the middle-dose and low-dose of Qi Ling Gui Fu Fang group had no significant difference(P>0.05).9. Compared with the control group, the amount of HIF-1α gene expression in vascular tissue of model group was significantly increased (P<0.05); Compared with the model group, high-dose, middle-dose and VC group in the vascular tissue HIF-1α gene expression were significantly decreased(P<0.05), the low-dose had no significant difference(P>0.05).In summary, select AA broilers and using low-temperature, high-fat, high-energy and high-sodium method, constructed the AS pathological model successfully. Qi Ling Gui Fu Fang can effectively prevention and cure AS, the mechanism is improving the body’s antioxidant capacity, inhibition of AS-related endothelial cells activity factor HIF-1α and its downstream regulatory factors VEGF overexpressing, protect endothelial cells. |
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