Breeding Of Three Lines And The Genetic Study Of Recessive Genic Male Sterile Mutation Material In Brassica Napus L

Because of the stable and complete male sterility, the comprehensive restorers and nonegative effect from the environment, rapeseed recessive genic male sterility is an importantway in rapeseed hybrid production.9012AB is a new type genic male sterility line which iscon-trolled by three genes.(Chen et al.,1993)Dong et al.(2010) showed that the male sterilityof9012A is con-trolled by two individual loci, i.e.Ms3and Rf. There are two alleles at the Ms3locus, the dominant wild type allele Ms3is the fertile allele, while its recessive allele ms3thesterile one. Three alleles are found at the Rf locus, individually Rfa(mutation restorationallele), Rfb(mutation sterile allele) and Rfc(wild type fertile allele), with the dominancerelationship of Rfa>Rfb>Rfc. In2004, there was a white single mutation material in Shaanxiprovince regional trial. Then it manifested the separation of fertility after self-crossed. Thismaterial was derived from the three recessive genic male sterile line bred by AnhuiAgricultural Sciences Institute. Found a white mutant plant, selfing produce sterile separation,successive generations siblings cross, in2009the No.628lines appear, siblings crossfertility segregation ratio of1:1in line with the material, after628consecutive lines4siblings post harvest fertility segregation ratio is1:1, while the corresponding self-fertileplants segregation ratio of3:1, indicating a stable number628strains homozygous two-typeline. Zhang Xuemei, etc. in2008numbered438strains obtained initially consideredtemporary insurance department,2010to2013, the fourth consecutive year the full438selffertile, while homozygous sterile plants with full hybrid sterility, indicating that the label438Department for the temporary protection system.2010and2011a total of2Select41different genetic background, high oil content low erucic acid, sweet glycosides years of fullself-sterile line and hybrid materials, extensive selection of quality restorers, results showedthat41materials can be restore fertility infertility material, the material has a wide range ofrestorers and complete recovery. Use of temporary maintainer line with the lab ’s originalmethod of restoring fertility test cross progeny separation7:1ratio appears to validate thethree types of recessive genic male sterile Brassica genetic patterns. Genetic verification, theauthor got the number for non-recovery strains h25and138, consistent with self61:3,7:1segregation ratio test cross, and the emergence of non-recovery ratio of7:1strains measuredby test cross progeny self-fertile plants and61:3ratio of non-recovery plants sterile plants from the progeny test cross method further validates selfing appeared7:1reliability. Thebreeding of three lines were built and its genetic mode was tested and verified. And progenytest cross method fertility segregation ratio of7:1appears to validate the three recessivegenetic model genic male sterile Brassica napus type system through the use of temporaryholding and restore the original laboratory.Some stable duplicate two-type line, temporarymaintainer line, fully sterile line, and restores lines was built. Code h25which fittedsegregation (7:1) was got. After many years research, our team have bred this kind of genicmale sterile line to realize three lines breeding hybrid.