Proteomics Analysis Of ’Zaosu’ Pear(Pyrus Bretschneideri Rehd.) And Its Red Bud Mutation

In this study, the fruit of ’Zaosu’ pear (Pyrus bretschneideri Rehd.) and its red bud mutation (’Redzaosu’ pear) fruit were used as materials, and the fruit were treated by natural coloring and bagging.Theseparation, identification and bioinformatics analysis of different proteins were done by the techonology ofproteomics.The purpose of this study were to find the key factors which were associated with red pearcoloration from the protein level, and to provide the important information for molecular breeding of redpear. The main results were as follows:1. The single fruit weight, fruit shape index, number of fruit dots, surface color changes andanthocyanin contents were determined in this experiment.The results showed that fruit bagging had noobvious effect on single fruit weight and fruit shape index, but light had obvious effect on fruit coloration.With the lingt time prolonging, fruit coloring gradually deepened and anhocyanin content of pericarpincreased.2. TCA/acetone precipitation, Tris-HCl aqueous solution, DTT method, phenol method and improvedphenol method were compared. In these5kinds of protein extraction methods, the result shows thatimproved phenol method is the most effective to prepare ripe pear skin protein sample. The gel has a clearbackground and fewer stripes by this method.3. Comparative analysis of different crude protein quantitative method has been taken, the resultshows that fewer samples used and quicker operate were founded in Nanodrop method。4.Protein sample were anaylsis by dimensional electrophoresis，79proteins were founded and71proteins were identified succeed. Success rate of identify was89.9%. They mainly related to energymetabolism, amino acid composition, such as resistance to stress, photosynthetic process.5. Function analysis and subcellur prediction were taken on the identified protein, The Funcitonanalysis results shows that21%difference proteins are associated with energy metabolism,18%differenceproteins are associated with amino acid composition,11%difference proteins are associated with stressrespond,10%difference proteins are associated with Transcription,8%difference proteins are associatedwith Photosynthesis,6%difference proteins are associated with transport,6%difference proteins areassociated with defense response to disease,3%difference proteins are associated with ubiquitin,17%difference proteins can not forcast their funcation. The subcellular localization prediction results shows that10different proteins are located in cytoplasm,7different proteins are located in chloroplase,5differentproteins are located in membrane,4different proteins are located in cytoplasm,4different proteins arelocated in plastid,2different proteins are located in nucleus,2different proteins are located in mitochondrion,2different proteins are located in cell wall,2different proteins are located in endoplasmicreticulum and the other34different proteins can not found their subcellular localization by prediction.6. Protein domain, superfamily structure prediction and transcription validation were taken on TCTPprotein（Translationally-controlled tumor protein homolog Q6）, MYB domain class transcription factor(Q7), isoflavone reductase related protein (Q26), WHY domain class transcription factor (Q27),60S acidicribosomal protein P0(Q30), ARF domain class transcription factor (Q65).7. Protein-protein interaction network analyzed were taken on different proteins.