Effect Of Lipopolysaccharide On The Metabolism Of Glucose And Fatty Acids In Dairy Goat Liver

In order to study the effect of lipopolysaccharide on the metabolism of glucose and fattyacids in dairy goat liver, two doses of LPS were administered by intraperitoneal injection forconstructing subacute liver damage and glucose&lipid metabolic disturbance model inpresent study, and has taken a series deeper exploration including some relevant blood indexmeasuration and detection of the transcription or expression of relevant key enzymes andtranscription factors in liver at48h after LPS stimulation. The results are as follows:1) During from0h to8h after LPS administration in two LPS groups, the blood glucoselevel became a peak and then returned back to normal; the triglyceride (TG) level wasdecreased; the urea level was increased; the total protein (TP) level has a decreasing trend.During from8h to24h, the glucose and TP levels were continuing continuously decreased,but TG and urea levels continued to rise. During from24h to48h, the glucose and TPlevels were rise up, but TG and urea levels have a decline.2) The transcription of key glycolytic enzyme genes, ADPGK, PFK1and PKlr weredown-regulated in LPS group’s liver at48h after LPS stimulation.3) The transcription of key gluconeogenesis enzyme genes, PEPCK1, G6PC, FBP1andACSS3were down-regulated, but PCB was up-regulated in LPS group’s liver at48h afterLPS stimulation. However, the protein contents of PEPCK1, G6PC and FBP1werechangeless; the ACSS3content decreased; the content of PCB was increased in LPS groupat48h after LPS administration.4) The transcription of key fatty acid synthesis enzyme genes, ACC1, FASN and SCD1were down-regulated in LPS group’s liver tissue at48h after LPS stimulation. Thetranscription of CD36and CPT1-α were up-regulated, but CYP4A11-like transcription wasdown-regulated.5) The transcription of SREBF1and PGC-1α were down-regulated, but LXR-α, PPARA andPPARG transcription increase was not significant in LPS group’s liver tissue at48h afterLPS stimulation.6) The transcription and expression of insulin receptor (INSR) and INOS were both reducedin LPS group’s liver tissue at48h after LPS stimulation. The glucagon receptor (GCGR)transcription decrease were not significant, however, its protein expression was changelessin the low dose LPS group but reduced significantly in high dose LPS group. Conclusion:1) The effect of LPS on the liver metabolism is dose-related, injected40μg/kgB.W. of LPS can significantly affect dairy goat liver glucose and lipid metabolism.2) Different effects of LPS on glucose and lipid metabolism due to the nature of the materialand the key enzyme vary, and the alteration of hormones and their receptors is also animportant factor affecting metabolism.3) The effects of LPS on glucose metabolism enzymes reflected in multiple levels ofregulation; as blood glucose lowering, high gluconeogenesis enzyme protein stabilitydemonstrated in favor of gluconeogenesis.4) The influence of LPS in the gluconeogenesis pathway derived from propionic acid wasgreater than from amino acids.5) After LPS stimulation, the alteration of energizing pattern showed reduced glucosemetabolism and enhanced fatty acid metabolism in gene transcription level.