Preparation Of Cryptotanshinone Lipidosome And Its Treatment Experiment On Rat Model Of Endometritis

Endometritis is an inflammatory disease of cows, which primarily caused by infection ofStaphylococcus aureus, Escherichia coli and Streptococcus agalactiae. Currently, antibioticsare the major treatment method of bovine endometritis. However, drug residues andpathogens resistant to antibiotics have become severe issues because of prolong use, evenabuse of antibiotics in livestock products. Using of traditional Chinese medicine to treatdisease becomes a trend. Cryptonshinone is an ingredient of salvia miltiorrhiza, with effectiveantibacterial and anti-inflammatory activities. Liposome has been widely used as carrier ofdrugs for improving the dissolution rate because of its stability, target quality and low toxicityquality. The studies included: the bacteriostatic activity of effective ingredients of Baicalin，Total Tanshinones, Crytotanshinone, Berberine and Propolis Flavone were studies in vitro;the preparation of cryptotanshinone liposome and its antibacterial activity against Escherichiacoli, Staphylococcus aureus, and Streptococcus; and the effects of cryptotanshinonelipidosome on rat model of endometritis. The results showed that：（1）Antibacterial activity in vitro: The minimal inhibitory concentration （MIC） andminimal bactericidal concentrations（MBC）of Baicaline, Cryptonshinone, Total Tanshinones,Berberine and Propolis Flavonoid against Escherichia coli, Staphylococcus aureus andStreptococcus in vitro were determined by using agar diffusion plate method and brothmicrodilution method. The results showed that there were different degrees of MIC of everyingredient on the three pathgens，among them Cryptonshinone showed the stronger inhibition.The MIC and MBC of Cryptonshinone against Escherichia coli, Staphylococcus aureus, andStreptococcus were512,16,64mg/L, and1024,32,128mg/L, respectively.（2） The preparation of cryptotanshinone liposome and its antibacterial activity againstEscherichia coli, Staphylococcus aureus, and Streptococcus: The best preparation conditionsof cryptotanshinone liposome were examed by orthogonal test, with the medicine: fat=1:20,lecithin:tcholesterol=4:1, reacting6min in30℃, the mean encapsulation efficiency was87.31%±1.45%; The grain size was concentrated distribution in176±5.65nm. Thecryptotanshinone liposome prepared according to the above conditions has high encapsulation efficiency and drug-loading rate, uniform shape and particle size, and easily to be repeated.Besides, the MIC of cryptotanshinone liposome against Escherichia coli, Staphylococcusaureus, and Streptococcus was512,8,32mg/L, respectively; which were1,2,2timesrespectively compared with cryptotanshinone.（3）The effect of cryptotanshinone lipidosome on rat model of endometritis: Ratmodel of edometritis was developed by stimulating with3%acetic acid for3days, theninoculated with0.2mL9×108CFU/mL of Escherichia coli, Staphylococcus aureus, andStreptococcus once a day for3days. There was no significantly difference of uterus indexbetween middle-, high-dose group of cryptotanshinone liposome and control group (P＞0.05), but the difference between middle-, high-dose group of cryptotanshinoneliposome and model group was significant (P<0.05). The levels of IL-1β, TNF-α and IL-6inuterine tissue were significantly reduced after treatment of cryptotanshinone liposome,indicated that there was an anti-inflammatory effects produced by cryptotanshinone liposome.