Snp Detection Of Four Porcine Genes And The Association Analysis With Feed Conversion Efficiency

Pork is the main source of meat in our country.The number of pork production and restock are increased year by year. nomatter how to improve the efficiency of feed conversion, reduce the cost of production, expand the scale of farming or realize the intensive farming has been the problem in pig production. With the development of life science and technology, pig disease resistance and production efficiency are improved significantly. But feed conversion efficiency is far short of expectations.Individual type, environmental factors and stress effect, feed factors, energy metabolism have different effects on feed conversion efficiency in swine. This study established an American Landrace resource population. Production traits be measured, combined with molecular biology, biostatistics and population genetics methods on production traits, Resistin, Orexin, CTNNB1, MC4R gene related testing, polymorphism, correlation analysis ADG, FI, PFI, FCR, IMF in pigs, verified the high and low residual feed intake differentially expressed genes screened by Digital Gene Expression Tag Profiling(DGE) By Real-Time PCR, yielding the following results:(1) An United States Department of Large White resources was established, including286castrates with a complete pedigree information and records of production traits and disease phenotypes recorded, extracted genomic DNA and RNA.(2) The locus974T>C was detected in Resistin gene. TT genotype was with higher ADG value than that of CC genotype(P<0.05).(3) The locus516G>A was detected in Orexin gene. The mutation site was significant associated with ADG, residual feed intake(RFI)(P<0.05), and significant associated with FI, PFI(P<0.01). GG and GA genotypes were with higher ADG value than that of AA genotype(P<0.05), GG and GA genotypes were with higher FI value than that of AA genotype(P<0.01), GG genotype was with higher RFI value than that of GA genotype(P<0.05).(4) The mutation site136520C>G and39464A>G were detected in CTNNB1gene. There was no available enzyme for the136520C>G, and the39464A>G was not significant associated with these traits(P>0.05). And no significant difference between the different genotypes(P>0.05).(5) The mutation site1426G>A was detected in MC4R gene. The1426G>A was not significant associated with these traits(P>0.05), no significant difference between the different genotypes(P>0.05).(6) Seven genes randomly selected from DGE for Real-Time PCR confirmati-on to verify the differentially expressed genes in high and low RFI. The results show that the expression trend of verified genes were consistent with DGE. And five genes differentially expressed in high and low RFI(P<0.05).