Development Of SSR Markers And Construction Of A Genetic Linkage Map In Mungbean (Vigna Radiate L.)

Mung bean (Vigna radiate (L) Wilczek) is an important food crop originated in China, and morethan2,000years of cultural history. Mung bean growing period is short, strong resistance, withnitrogen-fixing capacity, was among cereal, cotton, potato and other crops suitable for intercropping ofcrops, but also a good mitigation famine of catch crops. Mung bean are rich in protein, starch and low infat, are the ideal health food, but also for regulating the nutritional balance of the Southeast Asiancountries as an important nutritional supplement food. In addition, Mung bean can also be processedinto a variety of foods such as bean sprouts, mung bean wine, Mung beans and other food cake, Mungbean sprouts or people ingested an important source of vitamins and minerals. However, genetic studiesabroad for Mung bean and other crops is still lagging behind compared, molecular markers can beapplied to Mung beans are also very limited. This study was the use of bead enrichment methoddeveloped Mung bean SSR primers (Mung bean SSR) and Common bean SSR primers (Common beanSSR). According to the NCBI Mung bean genome sequence of pieces of information designed STSprimers (Mung bean STS) and select some transcriptome sequencing primers developed MGCP Mungbean (Mung bean EST-SSR) screening test. Finally, as the composition of recombinant inbred linesRIL12groups, using the above screening of molecular markers green genetic linkage map construction.The main results of this study are summarized as follows:1. Bead enrichment method developed by6100pairs of SSR primers Mung bean (Mung bean SSR),there are3,459pairs in the24Mung bean material can effectively amplify the effective amplificationrate was56.7%; number of polymorphic amplification primers559pairs, polymorphism was16.2%.Through the development of Mung bean transcriptome sequencing primers MGCP (Mung beanEST-SSR)149right, effectively amplified126pairs, the effective rate of84.6%was amplifiedpolymorphic effective amplification21pairs, polymorphism was16.7%; Bead enrichment methoddeveloped by Common bean SSR primers (Common bean SSR)424pairs,97pairs of effectiveamplification, the effective rate of22.9%was amplified polymorphic effective amplification six pairs,the effective amplification rate of6.2%; Mung bean STS primers (Mung bean STS)13right, effectiveamplification nine pairs, the effective rate of69.2%was amplified polymorphic effective amplificationtwo pairs, polymorphism was15.9%. Which, through bead enrichment method developed6,100pairs ofSSR primers Mung bean (Mung bean SSR), the trinucleotide repeat type SSR primers (Tri-nucleiotiderepeat), single nucleotide repeat type SSR primers (Mono-nucleiotide repeat) and dinucleotide repeattype SSR primers (Di-nucleiotide repeat) rate and the effective amplification of polymorphic rateshowed a gradual decreasing trend. 2.2669copies of the material for the Mung beans were insect identification experiment two years,were screened out of the Mung bean material with anti-sinking of621copies. This material seed621victims were less than≤20%. Insect screening out in the preliminary identification of this experiment621insect resistance in Mung bean material has high resistance to397to100%.3. In the United States a high sense of Mung bean weevil cultivar Berken (Vigna radiata ssp1radiata) and the Australian High Mung bean weevil resistant wild species ACC41(Vigna radiatassp.Sublobata) as the parental construct recombinant inbred lines RIL12, useing from6686pairs ofprimers screening out of588pairs in performance between the parents and the STS polymorphic SSRmarkers RIL12groups were analyzed to construct a containing585markers (499SSR markers,74RFLP markers,9STS markers and three RAPD markers) Mung bean genetic map, map length of732.9cM, including11linkage groups, the average distance between each mark is1.25cM, the averagelength of66.63cM. Each linkage group length of45.2-112.8cM, marked above each chromosomenumber is35-92, an average of53.18. Up marker loci containing92linkage groups LG1mark, a lengthof112.8cM; marker loci with minimal linkage group LG11contains only35marks, a length of48.7cM.4. The molecular genetic linkage map, the ACC41Bruchid resistant genes Br1positioned withinthe scope of the ninth linkage group a2.4cM interval, which is resistant genes Br1Bruchid SSRmarkers C220from both sides and from P2-627were used to0.7cM and1.7cM.