Characteristic Analysis Of NAC Transcription Factor Family And Correlation With Water Stress In Zea Mays L.

Drought has become a common problem faced all the world now, our country is the same,the drought is a major limiting factor of the world’s food security. Maize (Zea mays L.) is oneof the three major crops, china’s corn production is2.11million tons in2013, but abioticstress such as drought, high salt and high temperature seriously influence yield of maize. Plantformed defense system against adversity stress in adverse environmental after long-termsurvive, including transcription regulation of stress related gene expression is to adapt to theenvironment of the plant and the key factors in the process of resisting adversity stress.Studies show that transcription factors involved in the regulation of plant stress response, suchas AP2/ERF, MYB transcription factors, WRKY, bZIP and NAC, NAC transcription factor isa newly discovered type which plant specific transcription factor family. Some study has beenreported that NAC TF not only participated in the plant abiotic stress and biotic stressresponse, but also played an important role in the process of plant growth and development.This study preliminary analysis maize NAC transcription factors family and responsemechanism to drought stress of SNAC gene by analysis the evolution, conserved domains,predict membrane binding protein, chromosome distribution and stress experiment.Experiments used maize drought-resistant inbred lines as material, combined with thebioinformatics analysis, screen some stress response genes, namely SNAC (stress-responsiveNAC) genes.The main research results of this research are as follows:1. Ensure177NAC family members by multiple sequence alignment and phylogeneticanalysis to190，then constrcuted phylogenetic tree to177maize NAC family members and18homologous NAC sequences from oryza sativa and arabidopsis thaliana, the results showthat maize NAC family can be divided into two major categories, five subgroup NACⅠ,NACⅡ, NACⅢ, NACⅣ and NACⅤ. According to the physical location of NAC genes inthe chromosome, system nomenclature to190maize NAC transcription factors;2. On the basis of physical location and genetic position of NAC genes in maizeGDBdatabase, artificial draw the map of132maize NAC genes which coding177NAC proteinson chromosome;3. Predict the conserved motif, result show that maize NAC proteins has a highlyconserved NAC domain structure in the N-terminal, and the region is divided into fivedomain ABCDE, approximately consisting of150amino acid residues; C-terminal is unstabletranscription activation domain, these chatacteristics are consistent with the previous research. Predict the Membrane-bound NAC transcription factors show that there is ten membraneprotein in maize NAC transcription factor family, and13membrane area;4. Water stress treatment and gene specific expression analysis, results show that genehave different degree response to water stress in SNAC subgroup, and some correspondingSNAC protein have the motifⅤ[EVQS/QE/PK/IW/S] which the members of ATAF subgroupcontain.