Development Of DH Population And Molecular Mapping Of The Hybrid Necrosis Gene NetJingY176 In Aegilops Tauschii Using Microsatellite Markers

The rich unexplored natural variation of various Aegilops tauschii accessions can be easily exploited to improve common wheat using synthetic hexaploid wheat lines. However, hybrid necrosis, which is characterized by the untimely death of leaves or plants progressively, has been observed in certain interspecific crosses between tetraploid wheat and Ae. tauschii Coss.. The aim of this study was to construct a refined genetic map of a gene(temporarily named as NetJingY176) possessing hybrid necrosis in Aegilops accession Jing Y176.A triploid F1 population derived from the distant hybridization between Ae. tauschii Coss. and tetraploid wheat was developed to map the gene using microsatellite markers. The newly developed markers XsdauK539 and XsdauK561 were co-segregated with NetJingY176 on chromosome 2DS.The tightly linked markers developed in this study were used to identification of NetJingY176 among 91 Aegelipos accessions. Out of them, 49.45% of the Ae. tauschii accessions would carry NetJingY176, while other 50.55% of the Ae. tauschii accessions showed WT genotypes. Interestingly, hybrid necrosis genotypes tended to appear more in Ae. tauschii ssp. tauschii than in Ae. tauschii ssp. strangulata.