The Identification Of The Storage Protein In Some Wheat Lines From Huanghuai Wheat And Some Translocation Line Accessions Of The Haynaldia

Wheat is an important food crop with paramount importance. The improvement of wheat quality is now becoming one of the major tasks for the domestic breeding work due to the enhancement of individuals’ life in China. Considering that the content while composition of the proteins within wheat contribute to the wheat quality enormously, this study had used the SDS-PAGE, A-PAGE and HPCE technologies to analyze the storage proteins of the wheat accessions including both the new wheat varieties(or lines) from Huanghuai Wheat Zone and some translocation lines and addition lines accessions of the Haynaldia v.of Chinese spring. The variation of glutens of the Haynaldia villosa translocation line was analyzed and the quality assessment was conducted. Accordingly, the breeding work, especially that developed in the HuangHuai Wheat Zone was concluded and evaluated in order to select the appropriately reformed accessions from the Haynaldia villosa translocation lines. Ultimately, a part of Haynaldia villosa varieties whose properties are capable to serve as the cross parents and used as superior breeding materials are screened. The main outcomes of this study are follows:1. The T1 DL /1VS translocation line of Chinese-spring can be directly used for the quality improvement, however, the inside expression of the HMW-GS from Haynaldia v. was unstable. For the overall experimental materials, apart from the 1V/1D, the other translocation lines were all able to be applied in the real breeding without affecting the quality of gluten.2. After the analysis of the HMW-GS of the regional new wheat varieties(or lines) from Huanghuai Wheat Zone, it is reflected that most of these accessions occupy favorable subunits and high Quality scores, but lack the exotic subunits, suggesting that the breeding work in recent years do concentrate on the quality improvement whereas few extraneous varieties were introduced in the meantime.3. The fingerprint of prolamin of the regional new wheat varieties(or lines) from fingerprint showed that these materials could be divided as 6 groups in accordance with the coefficient of prolamin. And all the accessions were assembled as one cluster only when the coefficient was 0.48, indicating that the wheat germplasms used in nowadays breeding not only contain vast prolamin resources but also possess extensive genetic foundations.4. In terms of the prolamin clustering result of the regional new wheat varieties(or lines) from Huanghuai Wheat Zone, the first subgroup within the first cluster had presented a high coefficient of gliadins with Chinese-spring, particularly Xumai918, Bumai18 and Bumai0642. This implies that it might be reasonable to utilize the Haynaldia v. of Chinese-spring for the further hybridization, which could be a good approach to breed new varieties. Simultaneously, concerning about that in the Sixth group of which the prolamin components have displayed a certain specificity, thereby to choose this proportion of varieties to hybridize with the translocation line of Haynaldia v. of Chinese spring may be somewhat feasible to study the genetic and quality effects generated by the Haynaldia villosa under different inheriting circumstances.