Cytological And Molecular Marker Identification Of Wheat- Psathyrostachys Huashanica Translocation Line Metarial 24-6 And Establishment Specific SCAR Markers On Psathyrostachys Huashanica Chromosome 1Ns、3Ns、5Ns

Wheat is an important grain crop in China, it occupies an important position in the national life.Affinis wheat contains a large number of excellent properties and good genes which cultivar does’ t have,import those genes into wheat by distant hybridization,it is beneficial to widen the genetic background of wheat,and improve the existing wheat’s output,quality,disease resistance and stress resistance. Psathyrostachys huashanica Keng is one of the wheat species, belong to poaceae psathyrostachys, perennial cross-pollination plant,only grow in china’s Qinling and Hua mountain.Because it has cold resistance,drought resistance,poor soil resistance,precocity and other good qualities and good disease resistance.It is the genetic source of improving heredity and rarely resource of disease resistance.It caused full attention of agricultural scientists which depend distant hybridization with wheat.This research used morphologic observation,cytological detection,hybridization in situ to identify the breeding germplasm which crossed from common wheat variety 7182 and Psathyrostachys huashanica Keng.Hoping to provide theoretical basis for further research for Psathyrostachys huashanica Keng’s Distant hybridization breeding and utilization.Using molecular marker technology to develop SCAR markers for Psathyrostachys huashanica Keng’s chormosome 1Ns、3Ns、5Ns,In order to provide the molecular identification based on the hybrid offspring.Main results are as follows:1.Screening to an excellent agronomic traits of wheat- Psathyrostachys huashanica Keng translocation is 24-6.(1)Field observation showed that plant is compact, dark green leaves, grain white, half horniness, higher than the female parent strains is slightly lower, spike length, spikelet number per spike, spike grain number, and tiller number were increased;(2)Root tip cell observed article 24-6 chromosome is 42,2n=42;(3)To Psathyrostachys huashanica Keng Ns genomic DNA as probes, ordinary wheat 7182 genomic DNA as blocking, root and pollen mother cell in situ hybridization(GISH) analysis showed that the 24-6 carry 2 can arm of Psathyrostachys huashanica Keng pairs of chromosomes, to determine the 24-6 to stabilize wheat- Psathyrostachys huashanica Keng translocation system;2.Developed five huashan Psathyrostachys huashanica Keng specific SCAR markers:For accurately and quickly found the molecular markers of wheat- huashan new wheatgrass hybrids identification method,using 180 random primers(R1-R180) which length is 10 bp,To huashan psathyrostachys juncea, ordinary wheat 7182, Chinese spring wheat-Psathyrostachys huashanica Keng 1NS-7NS full two types additional department a total of 10 different materials for RAPD analysis.Got the Psathyrostachys huashanica Keng 1 Ns, 3 Ns chromosomes and 5 Ns, a total of five specific locus on a chromosome segments of RAPD molecular markers.Cloning and sequencing these specific RAPD fragments, after analysised the sequence, using primier v5.0 and oligo v7.0 for the SCAR primer design respectively, SCAR analysis was carried out on the 10 selected material again.Result showed two SCAR primers(S1-20 and S1-29)only amplify the specific bands on Psathyrostachys huashanica Keng and 1Ns full two types additional department,two SCAR primers(S3-113 and S3-125)only amplify the specific bands on Psathyrostachys huashanica Keng and 3Ns full two types additional department,1 SCAR primers(S5-3)only amplify the specific bands on Psathyrostachys huashanica Keng and 5Ns full two types additional department,the wheat cultivars are not amplified in the 7182 the corresponding bands.It showed S1-20 and S1-29 are Psathyrostachys huashanica Keng 1Ns chromosome’s specific SCAR markers,S3-113 and S3-125 are Psathyrostachys huashanica Keng 3Ns chromosome’s specific SCAR markers,S5-3 is Psathyrostachys huashanica Keng 5Ns chromosome’s specific SCAR marker.These Psathyrostachys huashanica Keng specific SCAR markers can be used for the detection of wheat under the background of Psathyrostachys huashanica Keng chromosomes, at the same time for wheat- Psathyrostachys huashanica Keng provided the foundation for molecular identification of hybrids.