Cry2Ab-transformed Arabidopsis Thaliana Enhances Its Insect Pest Resistance And Virulence Of HearNPV Against Helicoverpa Armigera (Lepidoptera:Noctuidae)

Arabidopsis thaliana is widely used as a model for transgene research. This thesis studied on the insect pest resistance of Cry2Ab-transformed Arabidopsis thaliana and its enhancement to the virulence of Hear NPV. We expect to explore a new approach to control H. armigera by the combination of transgenic plants and Hear NPV. Principal findings are following:1. The genetic transformation of Cry2 Ab gene was performed by Agrobacterium-mediated DNA transfer, and 7 putative transformants of Arabidopsis lines were selected by Basta. PCR analysis shows that the Cry2 Ab gene can be detected in the genomes of all these 7 lines.2. The quantitative RT-PCR analysis shows that Cry2 Ab express in line A, B, C, E, G. Among these 5 lines, line B has the highest expression level of Cry2 Ab, while line G has the lowest. There was no expreesion found in wild-type.3. Bioassay shows that H. armigera has the highest mortality(65.67%) when fed on line B. Mortality of the H. armigera feeding on line C was 50.33%, on line A was 47.33%, on line E was 46.67%, on line G was 42.67%.4. Enhancement trial shows that LT50 and LT90 of H. armigera when fed with line B and Hear NPV reduced 36.05% and 39.95%, respectively, as compared to the control. When feeding them with line G and Hear NPV, LT50 and LT90 reduced 17.96% and 25.67%, respectively.This research revealed that the Cry2Ab-tranformed Arabidopsis thaliana can significantly inhibit the development of H. armigera and enhance its insect pest resistance; Cry2Ab-tranformed Arabidopsis thaliana can significantly virulence of Hear NPV, shorten the lethal time of H. armigera.