Polymorphism Of TaSnRK2.10 And Its Association With Yield-Related Traits In Wheat

Protein phosphorylation and dephosphorylation is an important biochemical reaction of energy metabolism and signal transduction of plants when they are under abiotic stress, and is a regulation mechanism generaly exist in plant. The sucrose non-fermenting protein kinase (SnRK) is a kind of Ser/Thr protein kinase found widely in plants and participates a variety of transductions in signaling pathway in plants. TaSnRK2.10 is an important SnRK2 member involved in response to various abiotic stresses in wheat (Triticum aestivum L.). The objective of this study is to detect the single nucleotide polymorphism (SNP) of TaSnRK2.10, develop and map its functional markers, analyze the relationship between its haplotypes and phenotypic traits, and provide a basis for the genetic improvement and germplasm enhancement by molecular marker assisted selection in wheat.In this study, thirty hexaploid wheat accessions with high polymerphism, 4 tetraploid and 12 diploid wild relative species were chosen as plant materials. Analyzing single nucleotide polymorphism of TaSnRK2.10 gene by direct sequencing. A set of Chinese Spring nulli-tetrasomic lines and recombinant inbred lines (RIL) derived from a cross of "Yanzhanl×Neixiang 188" were used to map TaSnRK2.10-A on chromosome. The relevance between TaSnRK2.10-A haplotypes and phenotypic traits was analyzed using a natural population consisted of 262 historical wheat accessions. The purpose of the present research was to discover the favorable haplotype and favorable alleles. The main results are as follows:1. Two kinds of fragments were obtained from the genome of 6 hexaploid wheat accessions which were parents of DH lines (Hanxuan 10×Lumai 14), RILs (Opata×W7984) and RILs (Yanzhan 1×Neixiang 188). They were assigned on wheat genomes A and D, named as TaSnRK2.10-A and TaSnRK2.10-D.2. Seventeen variants in the entire region of TaSnRK2.10-A, comprising 15 SNPs and 2 indels, were identified among the 30 accessions with high polymorphism. No variant was found in TaSnRK2.10-D.3. There was no variant between Hanxuan10 and Lumai14, Opata and W7984 except Yanzhan 1 and Neixiang 188. Based on a SNP between Yanzhan 1 and Neixiang 188, TaSnRK2.10-A was mapped in the intervals between markers Xwpt7001 and WMC48 on chromosome 4A, with 5.1 cM and 25.7 cM from the flanking markers.4. Analyzing the sequence results of thirty hexaploid wheat accessions, they are clustered into four haplotypes. Using the CAPS markers developed for TaSnRK2.10-A detected the genotypes of the natural population, combined with phenotypic traits of three years for the association analysis. Four haplotypes were significantly associated with thousand grain weight, number of spike per plant, and number of spikelet per spike, respectively. The Hap Ⅱ and HapⅢ were the favorable haplotypes for thousand grain weight. The Hap IV was the favorable haplotype for number spike per plant. The favorable allele of high thousand grain weight is cytosine(C) at the position of 4184 bp.5. Construction rice vector with the genotype of TaSnRK2.10-A favorable allele.