Gene Mapping And Functional Aanalysis Of The Z37 Gene Thatcontrols Plant Narrow-leaf And Tiller Bud Outgrowth In Rice(Oryza Sativa L.)

Rice is one of the major crops in China, The physiological mechanism is a basic premise of rice genetics and breeding. Leaf shape of rice directly affect the efficiency of photosynthesis. the number of tillers number is directly affect the rate of seed. So leaf sheap and tillers are important agronomic traits for rice production. IAA is a important hormone in the plant, which play a broader role in the process of plants growth and development. As the complexity of biosynthesis, the plant hormone synthesis pathway contains several tryptophan-dependent pathway and tryptophan-undependent pathway. However, the molecular mechanisms and biological role of these pathways is still little known. The hormones transportion of plants depends on the vectors of IAA, and non-uniform distribution of auxin carriers in the cell membrane would lead to phytohormones polar transport. Polar transport of plant hormone involved in plant phototropism reaction, stretching growth and Photomorphogenesis reaction. More and more evidence imply that IAA are closely linked to rice leaf development and morphogenesis. What’s more, It have been reported that the content of IAA has improved in the highly-tillers plants. Therefore, some scholars suggested that IAA must be associated with and Strigalactone synthesis or metabolic pathways. In this paper, a highly-tillers and narrow-leaf mutant in rice was isolated from the rice T-DNA insertion population constructed by our lab, and named z37. Genetic analysis indicated that the z37 mutant is controlled by a single recessive gene. Using map-base clone we cloned the Z37 gene on the long arm of chromosome 1. The gene consists of two exons and one intron, encoding a lipase A protein. As the mutant was expressed narrow-leaf, dwarf and highly-tillers phenotypes, so we passed on tillering mutant z37 related genes and quantitative detection of IAA gene family. It find that D3, D10, D14, D53 and IAA1, IAA24 have increased in different degrees. Our laboratory were analyzed the biology agronomic traits statistics, genetics and molecular biology of z37 mutant in detail. And construct a complementary vector, overexpression vector, RNAi vector and other basic vectors, which laid the foundation for further clarification IAA regulatory mechanisms of signal transduction in the plant.