Different Expression Of Circulating MicroRNAs Associated With Schistosoma Japonicum Infection In A Final Host

Schistosomiasis japonica is a parasitic zoonosis caused by the Schistosoma japonicum. Although in China we made great achievements in controlling and curing this disease resently, but with the conditional changing like global warming there are more new challenges emerged. For better control of Schistosomiasis japonica, we need to use the modern molecular technology to research it and reveal the molecular level mechanism of infection by this parasite, will guide us to find a new way to control and cure this disease.In this thesis we can mainly divided it into two sections:1) analyzed the altered miRNAs in the plasma of infected rabbits with Schistosoma japonicum using the bioinformatics methods based on our previous Solexia data; 2) q RT-PCR method to further verify these altered miRNAs associated with schistosome infection. In brief:1. Circulating microRNA involved in much life process’ s adjustment, some specific circulating microRNA could performed like a biomarker to diagnosis some certain dieases. Our results indicated that there were 173 altered miRNAs being able to be co-detected in two biological replications.Further analyses indicate that totally 64 miRNAs are differential expressed in P1 and P2(P1:the pool constructed by plasma of infected rabbit after 14 and 28 days;P2:infected after 10、14、28、32days),12 miRNAs were down-regulated and 52 were up-regulated;60 miRNAs were detected only expressed in P1 and P2。191 Novel-miRNAs and 190 Novel-miRNAs were detected in the P1 and P2.Under the strictly conditions we selected the 18 miRNAs to predicte target genes, result shows that 416 target genes be predicted. Using those predicted genes we performed GO and KEGG analysis, target genes of these altered miRNAs are putatively involved in many biological processes such as cellular process, metabolic process, biological regulation, developmental process, and catalytic activity and so on.2. We used a qRT-PCR method to further verify these altered miRNAs associated with schistosome infection. We selected 10 altered miRNAs to perform the qRT-PCR, 9 of them were up-regulated only one was down-regulated, and our data matched Solex sequencing perfectly. The miR-10 were up-regulated, miR-10 demonstrated related to some cancer pathways, so this result will be a fundmantal for invesgating the mechanism of interaction bettwen host and Schistosoma japonicum.