Multifunction Of Marine Bacillus SP. On The Plant

468 strains bacteria that reserved in our laboratory were screened for the antimicrobial activity.Eventually took two strains from 468 strains,test the strain toenhance plant growth, and research the role to the growth of tomato,in stress tolerance of plants,prevention and control ofplant diseases.The result is as follows:1. Through morphology and molecular biology identification strain C217 and strain T28,starch respectively:Bacillus amyloliquefaciens and Bacillus sp..Will two marine bacillus strains on a variety of selective medium,the results showed two bacillus strains possesses nitrogen fixation,phosphate-dissolving, potassium-dissolving, siderophore production and 1AA production abilities. The vehicle production capacity of strain T28 and strain C217 are focated in a higher level,reaching 63.40%and 70.50%respectively.T28 strain ability of nitrogen fixation is better than strain C217,strain T28 possesses nitrogenase gene nifH and strain C217 don’tpossesses.2. Test strain C217 and strain T28 induced tomato seed germination of salt, The results indicated thattomato seed germination rate has declined obviously under salt stress,6.70% lower than control.Deal with by the two bacillus strains of tomato seed germination rate has different degrees of improvement,strain T28 processing tomato seed germination rate reached 81.53%, higher than the contrast by 11.16%.Strain C217 processing tomato seed germination rate reached 75.32%, higher than the contrast by 5.00%.3. The strain Bacillus sp. T28 possess multiple effects to the growth of tomato through biochemical and molecular biotechnology approaches. The results showed marked strain T28 obviously improved tomato seedlings chlorophyll content with increasement of 33.3% after successful colonization 15 days. The gene proB fragment was amplified from strain T28. These results suggested that strain T28 could adjust concentrations of pro line to induce plant salt tolerance. Strain T28 possess remarkable control effects on Botrytis cinerea and Sclerotinia sclerotirum, with the inhibition rates of 68.8% and 62.5%, respectively.