Genome-wide Prediction Of The Pht1 Family In Maize(Zea Mays B73)and Preliminary Identification Of The AM Specific-induced Transporters

Phosphorus is one of many nutrient elements which is necessary for plant growth and development. However effective phosphorus concentration in the soil far cannot satisfy the need of plant growth and development. High affinity phosphate transporters play a crucial role in the process of the plant efficient utilization the phosphorus in the soil. The formation of Arbuscular mycorrhizal is beneficial for the host plants on the absorption of phosphorus and its mechanism may be that infection of arbuscular mycorrhizal fungi will change the expression characteristics of some high affinity phosphate transporterin the host plant.So studing of the phosphate transportergene family and its relationship with arbuscular mycorrhizal fungi, have very important significance on corn production.This experiment used the methods of bioinformatics to predict the members of phosphate transporter gene phtl family in Zea mays B73. and made a further analysis of these phosphorus transport protein. To deal with the Zea mays B73 with low phosphorus nutrition, and analysised of phosphorus transport proteins expression patterns. In addition, the experiment that arbuscular mycorrhizal fungi sterile infect maize B73 root were done, and analysised of phosphorus transport proteins expression patterns between noncolonized root and colonized root. The results are as follows:1. Through a series of bioinformatics analysis,identified 92 nonrepeat candidate phtl genes that contains Sugart domain structure (Sugart domain is essential to phtl family proteins), finally. And to use bioinformatics tools analyzing the sequences of genes and proteins, constructing the phylogenetic trees.13 close relative phtl genes on the same branch. Sequence attribute, chromosomal location, gene replication and motif distribution of these 13 genes were further analysised.2. Expression type of different phosphorus transport genes in different tissues and organs type is a difference. All of the 13 genes expression in shoot tips and roots, some genes expression specificity in other tissues and organs.3. The analysis of expression pattern of low phosphorus treatment showed that:the expression quantity of ZPt1, ZPt3, ZPt 4. ZPt5 and ZPt8 were up-regulated as time goes, regardless of low phosphorus conditions or high phosphorus conditions; the expression quantity of ZPt1, ZPt3, ZPtS, ZPt8 and ZPt11 were up-regulated under low phosphorus conditions; the expression quantity of ZPt4 was basically unchanged under low phosphorus conditions; only the expression quantity of ZPt13 was irregular change.4. After arbuscular mycorrhizal fungi infection maize root, the expression pattern analysis of phosphorus transport protein showed that:After mycorrhizal formation, the expression quantity of ZPt1, ZPt3, ZPt5, ZPt8, ZPt12 and ZPt13 were down-regulated; the expression quantity of ZPt4, ZPt6, ZPt9 and ZPt11 were dramatically up-regulated, especially ZPt6 and ZPt11, their expression quantity were up-regulated about 100 times; ZPt2 and ZPt10 were not expression in noncolonized root, but were expression in colonized root. Analysis showed that these three genes can be identified as the mycorrhizal induced specific expression genes preliminary.