Single Marker Analysis On QTLs Underlying Yield-related Traits In Soybean Based On A Four-way Recombinant Inbred Lines Population

In this study we constructed using 160 F5:7 derived RILs from the four way cross（Kenfeng14×Kenfeng15）×（Kenfeng19×Heihe48）. From 2013 to 2014, Phenotypic value of important agronomic trait of 160 individuals was tested, which included pod number per plant, seed number per plant, seed weight per plant, 100-seed weight. Four yield related traits in a single marker analysis, aim at the soybean yield related traits of important QTL, analysis of QTL genetic effect, for soybean molecular design breeding to lay the theoretical and technical support.The main research results are as follows:1 In 2013 and 2014, 130 yield-related traits QTL were located under four environment contain Harbin and Keshan. The results are as follows: the number of 100 seed-weight QTL, pod number per plant QTL, seed number per plant QTL and seed weight per plant QTL is 48, 24, 12 and 46 respectively was detected respectively, and they distribute in 10 chromosomes of A2, C1, C2, D1 b, F, K, J, N, L and G, which belong to the 20 linkage of soybean. The inheritability ratio is 0.46%-8.75%,1.04%-11.62%,1.94%-3.33%,1.25%-8.25% respectively.2 In this study we found the Excellent alleles of pod number per plant were Satt281（Q2Q2）, BARCSOYSSR₀9₀183（Q1Q1）, BARCSOYSSR₀2₀849（Q2Q2）, Sat₁73（Q1Q1）, BARCS-OYSSR₀2₀607（Q1Q1）, BARCSOYSSR₁0₄44（Q3Q3）, Satt125（Q2Q2）,and the excellent alleles of seed number per plant were AI856415（Q1Q1）, BARCSOYSSR₁7₀160（Q2Q2）, Satt688（Q1Q1）, Satt529（Q1Q1）, BARCSOYSSR₀9₀183（Q1Q1）, BARCSOYSSR₁0 1444（Q1Q1）, the excellent alleles of 100-seed weight were Satt281（Q1Q1）, Satt173（Q3Q3）, BARCSOYSSR₁6₀566（Q1Q1）, BARCSOYSSR₁9₀451（Q2Q2）, Sat₃04（Q1Q1）, Sat₁73（Q3Q3）, the excellent alleles of weight per plant were BARCSOYSSR₀5₀513（Q1Q1）, BARCSOYSSR₀3₁620（Q2Q2）, BARCSOYSSR₀9₀183（Q1Q1）, BARCSOYSSR₀2 0607（Q1Q1）, BARCSOYSSR₀6₀011（Q1Q1）, Sat₃67（Q4Q4）, Satt338（Q1Q1）, BARCSO-YSSR₁7₀610（Q2Q2）.3 There are two sites control the four characters at the same time : AI856415, BARCSOYSSR₀8₀966; and There are four sites control the three characters at the same time, respectively Sat₁73, Satt281, BARCSOYSSR₁7₁633 and BARCSOYSSR₁1₀442; There are fifteen sites control the two characters at the same time, BARCSOYSSR₀9₀849, BARCSOYSSR₁0₁444, BARCSOYSSR₀9₀183, BARCSOYSSR₀2₀607, Sat₁53, Sat₂64, Satt193, Satt268, Satt462, Satt547, BARCSOYSSR₁9₀451, BARCSOYSSR₀5₁017,Satt292, Sat₃13, Satt193.