Drought Resistance Identificaton Of T5 Generationgs Of WK Transgenic Two Wheat(Triticum Aestivum L.) Species’ Lines

As one of the main crops in China, wheat is mainly planted in arid and semi-arid regions, and in this places drought is mainly abiotic case that limits the increasing and stability of wheat yield.With the development of transgenic technology, combining transgenic breeding and conventional breeding methods is a useful and effective approach that cultivate new drought-resistant wheat cultivars. The WK gene participates in conduction of adversity-stress signal, is of great significant for improving wheat’s ability to defense adversity. This study use WK transgenic lines, Kenong199 lines with WK and Jimai22 lines with WK, as test materials. On the basis of getting positive transgenic lines, combined with analysis on transgenic lines’ agronomic traits and yield, this study has comprehensively analysed on their physiological and biochemical indexes including chlorophyll content, relative water content, relative electric conductivity, soluble protein content,MDA content, antioxidant enzyme activity, photosynhesis rate and water use efficiency to comprehensively evaluate the drought-resistant performance of Kenong199 lines with WK and Jimai22 lines with WK.1.The results of physiological and biochemical indexes indicated that under drought stress the chlorophyll content, relative water content, soluble protein content, antioxidant enzyme activity,photosynhesis rate and water use efficiency of WK transgenic lines are all higher than no-transgenic ones at different growing stages, while their relative electric conductivity and MDA content are both lower than the control, which shows that the expression of WK gene can defense drought’s harm to plant’s normal physiological and biochemical reaction and metabolism, and can improve transgenic lines’ ability to adapt drought stress.2.The results of agronomic traits and yield showed that under drought stress the transgenic lines’ agronomic traits including plant weight, spike length, grain numbers per spike and1000-grain weight are all better than no-transgenic ones, and which indicates that transgenic lines have higher potential of improving field. Transgenic lines have higher plot yield compared to the control, the two positive lines 11W285-1 and 11SR1505-1 both exceed the no-transgenic one Kenong199 by 9.13% and 7.27% respectively. As another two positive lines, 12SR502-1 and12SR501-1 ‘s field exceeds Jimai22 by 10.15% and 2.93%. All these four positive lines belong to strong drought-resistant level. The results demonstrated that the transgenic wheat lines with WKshows a stronger resistance to drought stress than the no-transgenic ones.