| In this paper, we studied the effect of Glomus mosseae on the physiological growth of alfalfa, and the relationship with organic acids in plants under mixed saline-alkaline conditions. It provided a theoretical basis for the use of AMF resources to improve alfalfa quality and saline soil. Conclusions are as follows:1. Under natural conditions, the inoculated AMF treatment was higher than the non-inoculated group of alfalfa on plant height, total biomass, leaf area, chlorophyll a content, lower than the non-inoculated group of alfalfa on chlorophyll b and total chlorophyll content. Inoculation of AMF increased alfalfa leaf soluble protein content, proline content, soluble sugar content, POD activity, SOD activity. And reduce the content of alfalfa shoots ferulic acid content, salicylic acid content and protocatechuic acid content. Under natural conditions, AMF can promote the physiological growth of alfalfa significantly.2. With the increase of salt-alkaline gradient, alfalfa infection rate, root density and root arbuscular mycorrhizal colonization density decreased. Synergy high salt-alkali will destroy the structure of AMF and decrease mycorrhizal dependency of alfalfa.3. Whether or not AMF of inoculation, the survival rate, plant height, total biomass, leaf area, chlorophyll a, chlorophyll b, total chlorophyll content, soluble protein content and POD activity of alfalfa were significantly decreased with the increase of salt-alkaline degrees. But the proline, soluble sugar, SOD activity, ferulic acid, protocatechuic acid and salicylic acid content of alfalfa were significantly increased. Jasmonic acid was decreased with increasing salinity gradient, and the pH is greater than 8.45, jasmonic acid was not detected.4. Under salt-alkaline conditions, survival, plant height, total biomass, leaf area, the total content of photosynthetic pigments, proline, soluble sugar, soluble protein content, SOD activity and POD activity of these indicators, inoculated treatment group was higher than the non-inoculated treatment group of alfalfa in these indicators. AMF have a certain extent alleviate the inhibitory effect of salt-alkaline stress on plant growth, and maintain normal physiological metabolism of plant tissues, and improve the survival rate of plants.5. Under salt-alkaline conditions, It produce different effects of AMF on four kinds of organic acids. Salicylic acid, protocatechuic acid content of inoculatied alfalfa was lower than non-inoculatied alfalfa. Ferulic acid levels were unchanged. Jasmonic acid in the inoculated alfalfa was not detected. |
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