| The study, through feeding experiment, fluorescence quantitative and Western blot method, was to investigate the diet different NFC/NDF and protein levels in Inner Mongolia fine wool sheep rumen epithelial VFA absorption quantity of related gene expression and regulation of nitrogen transport.The contents and results of this research are as follows:Experiment 1 Effects of different NFC/NDF diets on digestion and metabolism, rumen environment and SCFA absorption of sheepSelected 9 good physical condition, body weight 43.8±4.75Kg with permanent rumen fistula sheep in Inner Mongolia Erdos half sib wethers, divided into 3 groups:NFC/NDF were 0.35,0.66 and 1.14, the same level of protein,13.5%. The results showed that dietary NFC/NDF had no significant effect on nitrogen metabolism of fine wool sheep. Fecal NDF with dietary NFC/NDF decreased. Rumen VFA increased with NFC/NDF increasing, the concentration of acetic acid with NFC/NDF increased, increasing the concentration of propionate and butyrate. The results showed that the expression of MCT1, PAT1, groups vH+ATPase gene had no significant effect (P> 0.05). Expression of NHE3 and DRA increased with the increase of NFC, but the expression of MCT4 decreased with the increase of dietary NFC. Expression of NHE1 and Na+/K+ATPase in the NCF/NDF is the highest at 0.66, its expression reduced while the NFC/NDF is too low or too high.Experiment 2 Effects of different NFC/NDF and RDP diets on digestion and metabolism, rumen environment and SCFA absorption and nitrogen transport in the rumen epithelialSelected 12 good physical condition, body weight 44.2±4.35Kg with permanent rumen fistula sheep in Inner Mongolia Erdos half sib wethers, divided into 4 groups by weight:group 1, NFC/NDF:1.14, CP:15.5; the 2 group,3 group NFC/NDF:1.14, CP:10.5; NFC/NDF:0.35, CP:15.5; group 4, NFC/NDF:0.35, CP:10.5. Feeding and management with experiment 1. The results show that the effect of fine wool sheep, nitrogen metabolism by dietary RDF, increased with the increasing of dietary RDP. But there was no significant difference between groups of fecal nitrogen and fecal NDF contents. Rumen pH with diet decreased with the increase of NFC/NDF. The concentration of rumen NH3-N increased with RDP increasing, and NH3-N concentration significantly increased while diet NFC/NDF decreased. Diet NFC/NDF and RDP had no significant influence on the expression of NHE1, Na+/K+ATPase and the vHPATPase rate (P>0.05). The expression of MCTland MCT4 were the highest while NFC/NDF and RPD were relatively high. Affected by diet RDP, the expression quantity of NHE3 increased with the increase of NFC/NDF. Low N diet can increase the expression of UT-B at the same level of Diet NFC/NDF phase. The expression of UT-B in low NFC/NDF group increased. While diet NFC/NDF was higher (1.14), the expression of aquaporin protein decreased with the increase of diet protein, while.diet NFC/NDF was lower (0.35), the expression quantity of water channel protein increased with the increase of the diet protein.While diet NFC/NDF was higher (1.14), the expression of ammonia transport protein decreased with the increase of protein diet, while diet NFC/NDF was low (0.35), the expression amount of ammonia transport protein increased with the increase of the diet protein.Experiment 3 The effect of feed restriction and week of recovery on ruminal NH3-N, pH and VFAExperimental animals, grouping and breeding management were the same with experiment 1. The results showed that:while the concentrations of total VFA was between 63.68 and 89.49mmol/L, it increased with the increase of NFC/NDF. While acetic acid concentration was between 48.35 and 65.75 mmol/L, it decreased with the increase of NFC/NDF.Except for 11:00, there was no significant difference between the concentrations of acetic and propionate acid. There were no significant differences in NH3-N concentrations among the groups (P> 0.05). The pH increased significantly with the increase of dietary NFC/NDF. The feed restriction after sudden recovery of the feeding was destroyed rumen environment, so that the rumen pH decreased. |
PDF Full Text Request