Study On Ultraviolet Fingerprint Of Dendrobium Officinale

Dendrobium officinale K imura et Migo, a herb from Dendrobium genus of Orchidaceae family, has been used as a good C hinese medicinal material from 2000 years ago, with the effect of tonifying stomach, promoting fluid, nourishing yin, clearing heat, antioxidant, tumor inhibition, decreasing blood sugar and boosting immunity. The wild populations of Dendrobium officinale decreased sharply due to the increasing market demand and excessive harvesting. Now the commercial products of Dendrobium officinale were cultivated ones. In order to evaluate the quality of the Dendrobium officinale products for the development of this industry, the ultraviolet fingerprint combined with chemometrics methods were used to analyze and assessment the quality of the Dendrobium samples. The observation of their agronomic characters and searching for relative literatures were also performed for the research of germplasm, adaptability regionalization, growth, and harvest time of Dendrobium officinale.The results showed that(1) Yuxi, Wenshan, Pu’er, Jinghong and Menghai of Yunnan Province are suitable for planting Dendrobium officinale. Among them, the contents of main chemical compositions of the plants from Wenshan and Menghai were higher than others.(2) Five germplasms with unique quality, disease resistance and high yields were selected from nine germp lasms.(3) January- March(before flowering) are the suitable period for harvesting Dendrobium samples. August is the critical period for the growth and compound conversion.(4) The model of ultraviolet fingerprint combined with partial least squares disc riminant analysis could identify Dendrobium officinale and nine relatives.(5) There were significant differences between the stems and leaves of Dendrobium officinale samples in ultraviolet fingerprint. Meanwhile, the ultraviolet fingerprint of the leaves varied due to the difference in geographical origins.