Identification Of Low Night Temperature Induced ABA Restrain Stomata Open Of MicroRNAs In Tomato Leaf

In the north of China in winter and spring,facilities vegetables vulnerable to low night temperature stress that inhibited photosynthesis of crops, and it is one of the key factors is to stomatal limitation factors associated with ABA. In recent years, miRNA has become a hot research, it as a control factor of a class of gene expression. The expression of genes related to effects of ABA signal pathway plays an important role in the regulation of stomatal opening and closure. Therefore, the study of miRNA in the ABA pathway plays an important role in the effect on stomatal limitation under the low night temperature (6℃). In this study, using wild type tomato (RR) and ABA deletion mutant (sit) as the test material under low night temperature (6 ℃), through high-throughput sequencing technology and biological information with qRT-PCR screening miRNAs which in the ABA pathway, to lay the foundation for elucidating the mechanism of miRNA effect of low night temperature stress induced ABA inhibited stomatal opening.This study obtained the main results were as follows:1 ABA content of wild type tomato control(RR/15℃)and low night temperature treatment conditions(RR/6℃)is higher than that of ABA deletion mutantRR, in a certain processing time, ABA content of wild type tomato and ABA deletion mutants were significantly increased and higher than that of the control level of the respective. In addition, the content of ABA increased night temperature in wild type tomato amplitude is higher than that of ABA deletion mutantRR, especially in low night temperature treatment of 3 h. The results show that, low night temperature stress induced the synthesis of endogenous ABA to adapt to environmental stress.2 Through the stomata ultrastructure observation results show that after the low night temperature treatment wild type tomato reduced stomata opening; While deletion mutant tomato leaf stomatal opening is also reduced after dealing with 7d. The results showed that ABA can significantly reduce the tomato leaf stomatal opening strength and opening rate to improve resistance of plants.3 This experiment adopts solexa high-throughput sequencing technologies sequencing the four small RNA libraries.Preliminary screening differentially expressed miR156/160 a/162/166b/1919/5300 and forecast novel-m0005/m0088-3p after statisticsing significant difference expression of known micrornas between the two samples. And through the qRT-PCR verify differentially expressed micrornas, its results consistent with high throughput sequencing.4 By the use of the Mireap software predicting the target genes of miRNA. miR159. miR166 expression of its target genes involved in low night temperature stress and showed sensitive to ABA, through participation in the signal transduction pathway induced stomatal closure, improve the anti stress ability of the night.The specific function of miR156/160/162/397/1919/5300/novel-m0005-3p/novel-m0088-3p target gene is not clear, but in this study, the miRNA response to the ABA induction, played an important role in the process of abiotic.