| Arabinogalactose protein (AGPs) is a kind of Highly glycosylation protein molecules which is rich in hydroxyproline/proline,and usually constituted by core protein chains and carbohydrates, AGPs typically contain>90%(w/w) carbohydrates, molecular weight in the60-300kd, they expression in leaves, flowers,roots,stems,and seeds At the subcellular level, AGPs are located on the plasma membrane and in the cell wall. In addition to their predominant localization at the plant cell surface, AGPs have been detected in the extracellular space.large researches show that the AGPs’ function is various, it is involved in cell proliferation, cell expansion and extension, programmed cell death, cell activities such as microspore development and cell aggregates.In this paper, rape microspore was used as material to observe the distribution of arabinogalactan proteins (Arbaniogalactna poretnis, AGPs) in the different periods of anther by immune localization; Secondly, the distribution of AGPs was investigated in embryonic development; Finally, the reagent βGlcY was employed to explore the biological function of AGPs in rapeseed microspore and zygote embryogenesis respectively. The main experimental results were as follows:(1)Using different periods of anther do paraffin section, through immune localization and aniline blue staining, compared the distribution characteristic of Arabingalactose protein between of several chapiter, we found that there are some difference in the two kind of slices’AGPs(2)Using manual separation technology to get different development period of the zygote embryo, observed their morphologic character, and treat different periods’ zygote embryo with different antibodies for immunofluorescence localization, observed the distribution of Arab galactose protein, the results show that the AGPs identified by the JIM4, before4cell proembryo, the green fluorescence appears in the whole body and suspensor surface, after zygote embryo develops into small globular embryo, the green fluorescence disappear in the body,and the suspensor expression is weak, but the end of the suspensor cells near the micropyle side express strong.JIM8recognition AGPs also have their own characteristics in the zygote embryo development, before4cell proembryo, JIM8recognition AGPs distribution pattern and JIM4recognition of AGPs distribution patterns are similar,,after the zygote embryo development to small globular embryo, the distribution of the embryonic body AGPs also disappeared, and had a higher expression in the suspensor, but1-2suspensor cells which connected to the body without green fluorescence.JIM13recognition AGPs throughout the zygote embryo development are expressed.(3)Establishment of microspore embryo culture system, and pick out the globular microspore embryogenesis in reagent αGlcY concentration is80microns NLN-13medium culture, similarly pick out the globular zygote embryo in the same concentration of culture medium, compared the process of embryo development in this two kind of system, the results showed that the microspore embryogenesis surface appeared a lot of fluffy structure, a small number of microspore embryogenesis deformation and appear a lot of hair structure in the surface, but most of the embryo to maintain the original state, their body are red; For zygote embryo, there is no root hair shape of structure or fluffy structure, but development is slower, it indicates that the function of AGPs in the two systems may be different, although microspore system can very good simulation of the zygote embryo development, but there are even have some different between the two kind of system. |
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