| Cyperus esculentus, commonly known as nutsedge, is a member of the Cyperaceae family. Nutsedge is root crop which grows mainly in the African coastal lands. Tubers of nutsedge are rich of oil, starch as well as sugar, with protein and various vitamins. This crop is adapted to different adverse environments and has been cultivated widely and readily, nutsedge is becoming a novel quality oil crop because of its extensive application range and enormous economic values.In plants, the synthesis of starch occurs by utilizing ADP-glucose as the glucosyl donor for the elongation of α-1,4-glucosidic chains. The first committed step in these pathways is the synthesis of ADP-glucose in a reaction catalyzed by ADP-glucose pyrophosphorylase(ADPGlc PPase, abbreviated as APS). The expression level of AGPase gene is of great importance in terms of accumulation of starch. WRI1is a member of plant specific transcript factor family designated as AP2/EREBP family, which is required for accumulation of TAG. WRI1directs carbon flow into oil accumulation by controlling genes involved in glycolysis and fatty acid synthesis.In this article, we cloned cDNAs of genes encodes AGPase and WRI1from nutsedge by RACE technology. The cDNA of APS and WRI1is1696bp and1423bp, which contain an ORF of1536bp and1098bp respectively. Sequence analysis showed WRI1and APS cDNA encodes a41.3kD and56.1kD protein respectively. The CDD search indicated that WRI1belongs to AP2superfamily with two AP2conserved structure domain, N terminus is lack of S. Core fragment of APS protein showed enzyme activity of AGPase, which is a member of glu-trans family A. Meanwhile we established WRI1overexpression vector and AGPase RNAi vector directed by35S promoter. |
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