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Study On Ruminal And Intestinal Digestibility Of Feed Protein And Amino Acids For Dairy Cow

Posted on:2015-07-09Degree:MasterType:Thesis
Country:ChinaCandidate:Y F SuFull Text:PDF
GTID:2283330467462907Subject:Breeding
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Twelve feedstuff samples for dairy cow were collected. The nutrive contents wereanalysised. The digestibility characters of samples in the rumen and intestine wereseperately measured by nolon bag techniqe and modified three-step method in vitro. Thestudy enriches the database of nutrive value of feedstuff for dairy cow and supportes thescientific ration mixed.1. Partial amino acid composition of12feedstuff were measured. The resultshows that the contents of methionine, lysine, histidine, phenylalanine, isoleucinecontent were the highest in protein feed (soybean meal,0.66%,3.14%,1.38%,2.22%and2.92%; cottonseed meal,0.86%,2.95%,1.57%,3.09%and2.76%; rapeseed0.71%,2.39%,1.18%,2.00%and2.65%; flax seed meal,0.68%,2.26%,1.33%,1.86%and2.59%; DDGS,0.66%,0.79,0.67%,2.94%and3.99%, DM basis). The content were lessin energy feed (corn0.17%,0.37%,0.21%,1.05%and0.67%; wheat0.14%,0.35%,0.52%,0.87%and0.95%. wheat bran,0.15%,0.78%,0.70%,0.82%and1.07%, DMbasis). The amino content were the lowest in roughage (whole corn silage,0.16%,0.34%,0.13%,0.40%and0.39%; Chinese wildrye,0.10%,0.23%,0.11%,0.62%and0.25%;Alfalfa,0.35%,1.32%,0.44%,1.01%and1.22%; Peanut vine,0.12%,0.24%,0.13%,0.49%and0.29%, DM basis).2. Three dairy cows with permanent ruminal cannulas were used to investigateruminal digestibilities of DM and CP by nylon bag technique. The ruminal digestibilities ofDM and crude protein were seperatelly76.86%and67.65%for soybean meal,49.70%and63.65%for cottonseed meal,67.73%and54.54%for rapeseed,51.21%and20.53%for flax seed meal,62.02%and24.41%for DDGS,60.06%and35.35%for corn,87.56%and72.73%for wheat,68.88%and79.04%for wheat bran,62.14%and53.89%for whole corn silage,30.87%and23.24%for Chinese wildrye,66.95%and78.84%for alfalfa62.27%and57.18%for apeanut vine.The ruminal digestibility of Met and Lys were different between feeds, even in thesame kind of feeds.The rumen digestibility of Met in most feed is greater than that oflysine.3. The residue was collected after cultured in rumen for16h for measuring theintestinal digestibility of DM and CP of feed by the modified three-step in vitroprocedure. The intestinal digestibility of DM and CP were seperatelly56.42%and96.18%for soybean meal38.31%and88.12%for cottonseed meal,34.38%and89.42%for rapeseed,52.53%and86.91%for flax seed meal,47.09%and89.55%forDDGS,76.30%and88.64%for corn,22.97%and78.01%for wheat,9.52%and63.63%for wheat bran,12.42%and50.90%for whole corn silage,14.97%and68.90%for Chinese wildrye,16.57%and66.66%for alfalfa,10.28%and54.33%for peanutvine.The results show that the ruminal degradability of DM and CP have the differenceamong feedstuffs.The feed reidue of rumen have the different potential capacity ofprotein supplying for intestine.The intestinal digestibility of Met and Lys is different among feedstuffs, even inthe same kind.The intestinal digestibility of Met in most feed is greater than that oflysine..
Keywords/Search Tags:cow, in vitro procedure, protein, modified three-step in vitro procedure, rumen digestibility, intestinal
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