Study On The Effects Of Enterococcus Faecium Application In Weaned Piglets Diets

In this study, the first choice to plant10commercially available lactic acidbacteria, by vitro antibacterial test on Escherichia coli (K88) to select a stronginhibitory effect of four strains of lactic acid bacteria, and then to study thebiological characteristics of the four strains of lactic acid bacteria, biologicalcharacteristics obtained over the good follow-up study of Enterococcus faecium.Under normal conditions, further study the impact of Enterococcus faecium growthperformance of weaned piglets and the effect of LPS stimulation in Enterococcusfaecium weaned biochemical, inflammatory and immune factors, antioxidantcapacity and intestinal absorption function and other related indicators, furtherstudy the effect of the application of Enterococcus faecium, in order to clarify themechanism action of Enterococcus faecium. The study is divided into the followingsections:Experiment1: Comparison of the biological characteristics of lactic acidbacteria strain analysis.First choice of ten commercially available lactic acid bacteria, Escherichia coliby (K88) in vitro antibacterial test to select the four strong inhibitory effect oflactic acid bacteria, and then select out of the four lactic acid bacteria resistance,resistance to bile salts, high temperature test comprehensive comparative analysisto select the best strain and fermentation probiotics. The study found that strains ofenterococci R1feces has strong antibacterial resistance, acid resistance, bileresistance and heat resistance, can be used as an excellent strain for subsequentfermentation tests.Experiment2: Enterococcus faecium impact on growth performance of weanedpiglets.The test select about21days of duroc×large white×landrace24crossbred pigswere randomly divided into three groups, each group of eight repeated, eachrepetition a pig. Two treatments were control group(basal diet), the experimental group (basal diet added6.5×106cfu/g of Enterococcus faecium). Feeding trial for21days, determine the growth performance of piglets.The results showed: Dietary with Enterococcus faecium can significantlyreduce the weight of15-21d piglet feed ratio (P<0.05), improved the weaning dailygain, feed weight ratio trend (P>0.05), diarrhea was significantly lower (P<0.05),described Enterococcus faecium can improve feed conversion rate, and effectivelyreduce the rate of diarrhea.Enterococcus faecium affect the test three of piglets blood biochemistry,inflammatory and immune factors, antioxidant capacity and intestinal absorptionfunction and other related indicators under LPS stimulation.Select the test about21days of duroc×large white×landrace24crossbred pigswere randomly divided into three groups, each group of eight repeated, eachrepetition a pig. Three treatment groups were control group(basal diet,saline),immune stress group (LPS group, basal diet, injection LPS), the experimentalgroup (Enterococcus faecium group based diet supplemented with6.5×106cfu/g ofEnterococcus faecium, injected LPS). The first21days in the test injection LPS(100μg/kg BW) or the same dose of saline,3h after injection of blood LPS. In thefirst21days trial period, after injection of LPS6h slaughter of all pigs, take theorganizations to be tested.These studies shown that:(1) Compared with the control group, LPS group increased the plasmaaspartate aminotransferase and total bilirubin (P<0.05); compared with the LPSgroup, dietary with Enterococcus faecium aspartate aminotransferase alleviateupward trend (P<0.1), total bilirubin levels were significantly decreased (P<0.05).(2) Compared with the control group, LPS group increased the plasma cortisoland plasma insulin-like growth factor (P<0.01). Compared with LPS group, dietarywith Enterococcus faecium improves liver plasma cortisol and interleukin-1βlevels (P <0.01), decreased the levels of prostaglandins (P<0.01).(3) Compared with the control group, LPS group decreased pancreatic CAT activity (P<0.05), increased the plasma MPO activity (P<0.05). Compared withLPS group, dietary supplementation of Enterococcus faecium improves pancreaticCAT activity (P<0.05), reducing the pancreas tNOS activity (P<0.05), liver CATlevels (P<0.05) and liver SOD activity (P<0.1).(4) Compared with the control group, LPS group decreased plasma D-xylose(P<0.01), jejunum alkaline phosphatase, invertase and lactase (P<0.05). Comparedwith LPS group,dietary with Enterococcus faecium improve the trend of jejunallactase (P<0.1).Conclusion: Dietary with Enterococcus faecium can reduce the F/G and diarrhearates of weaned piglets. Enterococcus faecium has beneficial effects in alleviatingLPS-induced passive effects in weaned piglets..