| The thaw-freeze stress caused by global warming, has greatly resulted in decreasingof output in agriculture、gardening, animal husbandry. It directly affects on thesustainable development of agriculture.NO and H2O2is well known to be thought asimportant chemical in increasing plant ability of resisitance to cold, drought, salt, andthe seed germination rate. To reveall the relationship between the NO and H2O2and theplant thaw-freeze resistance, and to understand the reasonable use dosage, it willcontribute to maintain productivity of agriculture and animal husbandry. We canexcavate its potential for the service of agriculture and animal husbandry, and providethe theory basis for chemical reagent use in improving the potential of winter wheatthaw-freezing resistance.Winter wheat is an important crop in northern China. Yield ofwinter wheat directly affects the lives of local residents and yield in agriculture.In this experiment, winter wheat was selected as a material, and treated bydifferent concentrations NO (select SNP as donor) and H2O2for6days. Then winterwheat treated by NO and H2O2were exposed to thaw-freeze stress. Tthe growthpotential of winter wheat were measured during treatment of different concentrations ofNO (to sodium nitroprusside (SNP), H2O2.The mortality rate and wilting rate weredetacted during thaw-freezing. Also, lipid peroxidation, antioxidant enzyme activity andosmotic adjustment content were investigated during thaw-freeze stress. The resultesobtained was as follows:（1）In the thaw-freeze stress, POD activity in the leaves of the winter wheattreated by NO had31.5%higher than the control group,16.7%higher than in thefreeze-thaw phase. CAT activity in the leaves of the winter wheat treated by NO hadaverage50.4%higher than the control group in the thaw-freeze phase,54.2%higherthan in the freeze-thaw phase. Soluble protein content in the thaw-freeze phase average20.7%higher than the control group. Proline content average208.8%higher than thecontrol group in the thaw-freeze phase,average37.9%higher than the control group inthe freeze-thaw phase.It can be concluded that NO treatment promoted the POD,CATactivity of antioxidant enzymes in the leaves of winter wheat, increase the content ofproline and soluble protein,to melt the frozen stress resistance ability of the winterwheat. （2）POD activity in the winter wheat treated by H2O2had higher by average12.6%than thecontrol group in the thaw-freeze phase,was higher by5.3%than the control group in the freeze-thawphase.CAT activity in the winter wheat treated by H2O2had higher by average43.5%than thecontrol group in the thaw-freeze phase, was higher by45.0%than the control group in thefreeze-thaw phase.Soluble protein content in the thaw-freeze phase had average31.0%higher thanthe control group, was slightly lower by10.7%than the control group in the freeze-thawphase.Proline content had higher by average155.2%than the control group in the thaw-freezephase,was higher by103.6%than the control group in the freeze-thaw phase. It can be concludedthat H2O2treatment promoted the POD, CAT activity of antioxidant enzymes in the leaves of winterwheat,increased the content of proline and soluble protein, and melt the frozen stress resistanceability of the winter wheat.The study showed that H2O2and NO can improve the ability of cell membrane lipidperxidation by raising the POD and CAT activity,and improve the ability of cells tomaintain moisture by increasing osmoregulation content (proline and soluble protein),then improve the winter wheat thaw-freeze stress resistance.Therefore, we can syntheseanalogues like H2O2and NO in the future, on the basis of not polluting the environment,to improve the antifreeze of crops and forestry thaw-freeze resistance.It has a certainapplication value in maintaining the sustainable development of agroforestry. |
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