Isolation And Identification Of Endophytic Fungi In Oxytropis Glabra DC On The Southern Slope Of Tianshan

Within Oxytropis endophyte refers in Oxytropis within a certain period of time or during the entire lifeliving in Oxytropis flowers inside plant tissues,not cause obvious symptoms of the disease a class of fungi.The study found that endogenous fungal secondary metabolites contains many biologically activesubstances.Southern Tianshan Mountain Akqi widely distributed in Oxytropis, is a typical temperate continental aridclimate, long summer without winter, spring follow autumn, connected to a large temperature differencebetween day and night, in more with the local Oxytropis splendens gregarious together to form a smallpiece of meadow grass populations of other rare. Etc. Oxytropis splendens strong resistance and becomethe dominant species of plants, which may be related to Oxytropis mutualistic endophyte and its a veryimportant relationship, because eating the local livestock will locoweed other similar cause toxic pastureevery year large quantities of poisoning deaths, deformities and infertility and miscarriage female fetuseslivestock, sire decreased sperm quality, livestock feed poisonous prairie livestock caused huge economiclosses irreparable. Research shows that the production of toxins and their metabolites of the endophyticfungi related to study locoweed poisoning has become a pressing issue.Oxytropis is the main poisonous plants in the southern slope of Tianshan region.In order to study thediversity of the southern slope of Tianshan Oxytropis endophyte, The method for sterilization of plantmaterials surface with75%ethanol and20%NaClO was determined by the general method of endophyticfungus’isolation and purification,and the species identification by the morphological method andrDNA-ITS for series isolated strains.It results: Four endophyte were isolated by PDA medium.Combinedwith "Fungal Identification Handbook" for morphological characteristics and molecular biology of fungithe strains XJ-AHQ-1is Schizophyllum commune, the strains XJ-AHQ-2and XJ-AHQ-4is Incertaesedis,phoma glomerata,XJ-AHQ-3is Tuherculariaceae,Fusarium,Fusarium triciuctum.Strains with theeffective publication similarity in95.8%~99.8%.The results show that the southern slope of Tianshanregion has more resources endophyte species.