Research And Analysis Of Long Non-coding RNA Expression Spectrum, A Co-factor For Prion Disease

[Background] Prion disease is a kind of neuropathic disease with high pathogenicity andlethality. It is caused by PrPSc, an aggregated moiety of the host-derived membraneglycolipoprotein-PrPc, accomplished by disordered cellular metabolism and mass mortality ofnerve cell. Now, it has been proved that human would infect Creutzfeldt-Jacob disease (CJD) ifintake some beef contaminated with MCD (mad cow disease). Thus, we can conclude that priondisease has broken the barriers of the genus and has also become a kind of life-threateningamphixenosis. After researchers’ intensive study, there is proved to be some host factors insidecelsl, which might have relationships with infections and pathogenicity of prion disease.Recently, scholar have found a type of long non-codind RNA with a length beyond200nt,which has considerable relationships with some life progress and might be the co-factor ofprion protein (PrP). Therefore, we make screening and identification of PrP-related LncRNAs.[Method] In our experiment firstly established the gene expression profiles of LncRNAand mRNA of brain tissue from the mouse model of PrPsc. After primary screenning, we firstconfirm the PrPsc-related LncRNA, then make out the target direction of functional LncRNA inthe process of prion attack. Finally, RT-PCR and western blotting are used to quantitativelyanalyze the functional LncRNA effect on the replication of prion gene and protein.[Result] According to the identification of RT-qPCR, we find that there are decrease of6LncRNA s’expression compared with the control group and increase of another13LncRNA.Among the mRNA chips of hippocampal tissues, there are170differently expressive mRNA,25of whose length are decreased, the others of whose length are rising. The target gene ofdifferently expressive LncRNA can be confirmed by analysis and prediction of bioinformatics.The analysis of RT-PCR reveal that the over expression of LncRNA-2doesn’t have obviouseffect on prnp gene, and the result of western blotting indict that the over expression ofLncRNA-2can inhibit the replication of PrPSc.[Conclusion] Based on the above experiment, we can conclude that the over expression ofLncRNA-2can inhibit the replication of PrPSc.It supply us a theory support for the furtherstudy of LncRNA and prion molecular chaperone.