Differential Proteomic Analysis Of The Transcription Factor Ss-Nsd1and Ss-FoxE1in Sclerotinia Sclerotiorum

Sclerotinia sclerotiorum(Lib) de Bary is a devastating fungal pathogen whichinfects various of economic crops. Sclerotium that produced during the sexualreproduction impose severe yield losses to crop industry and also determines theincidence of its epidemic sclerotium which is the main source of the primary infection,sclerotium plays the central role in the whole life cycle of S.sclerotiorum, sclerotiumgerminates forming sclerotium or mycelia, which together develop into the maturesclerotium, from which countless ascospores are released to initiate the reinfection.The sufficient ascospores allows a long-distance distribution and also raises thedifficulties for disease control. Therefore, this research engaged to analysis theS.sclerotiorum transcription factor knockout strains and wild type strains on the levelof protein, to explore the differentially expressed by transcription factor during theprocess of the formation of apothecium, which provides a theoretical basis forpreventing sclerotium diseases effectively.Our team get the Ss-nsd1gene completely knockout strain by the methods ofPEG mediated, which affects the integrity of the structure of sclerotium developmentand the formation of ascogonium during the germination process, which then leadingto that it can’t form apothecium. Based on the preliminary work, we compared theexpression difference between Ss-nsd1gene knockout strain and its wild type strain inproteomic level by two-dimensional electrophoresis, finding out that there are43protein spots whose expression abundance changes is above3times. Of these43protein spots,18%are related to energy production,7%are related to metabolism,5%are related to protein fate,4%are related to stress response,2%are related to cellgrowth and division,2%are related to cellular organization，the remaining arepredicted and hypothetical protein. Among them,18%are related to energyproduction, shows that Ss-Nsd1transcription factor significantly affect the energy production and then affect the formation of apothecium.The early stage of the study by our team shows that Ss-FoxE1transcriptionfactor is associated with sexual development, to analysis the whole protein of thedifferent between Ss-foxE1gene knockout strain and its wild type strain bytwo-dimensional electrophoresis, the result shows that there are42protein spotswhose expression abundance changes is above3times. Of these42protein points,21%are related to energy production,15%are related to cellular organization,9%arerelated to metabolism,9%are related to protein fate,6%are related to cell growth anddivision,6%are related to stress response, the remaining are related to predicted andhypothetical protein. Among them,21%are related to energy production, whichshows that Ss-FoxE1transcription factor is significantly affect the energy productionand then affect the normal form of apothecium.This research firstly to analysis the function of these two transcription factorsSs-Nsd1and Ss-FoxE1, providing an important basis on the analysis of differentexpression by the formation of apothecium on these two transcription factors, whichlays the foundation to further reveal the proteins related to the formation of theS.sclerotiorum apothecium.