Influence Of Arachis Hypogaea LPAAT And LEC1Respectively Over-expression On Oil Content In Brassica Napus L

Gene LEC1(Leafy Cotyledon1) is a transcription factor playing a key role in the process of embryonic development, and it can be combined with CCAAT-Box to activate downstream gene expressions. Gene LPAAT (Lysophosphatidic Acid Acyltransferase) is a functional gene that can catalyze LPA to PA. It is also a potential limiting step in the process of the TAG assembly, and a key enzyme of fat synthesis pathway in plants. LEC1and LPAAT play important roles in the plant fat synthesis, plant expression vectors containging bar gene, napin promoter, AhLEC1and AhLPAAT genes from peanut were introduced vector into Agrobacteria EHA-105and then used for rapeseed transformation. TO generation of transgenic plants and their offspring (T1, T2) were identified PCR-positive and also confirmed by southern blot. Oil content and fatty acid composition of T1seeds were analyzed by infrared scanning and gas chromatography. It was found that the oil content of genetically modified oilseed was not significantly increased, but its fatty acid composition changed significantly. Especially, the composition of oleic acid and linoleic acid increased significantly, while the erucic acid content was reduced significantly. Therefore, the ectopic expression of AhLPAAT gene and AhLEC1gene in rapeseed can produce stable genetic phenotypic changes in the transgenic lines.