| Sucrose synthase（Sus）, a key enzyme in sucrose metabolism, plays an important role in both plantgrowth, development and osmotic adjustment. In cotton, the gene has been confirmed to be related tocotton fiber development, however, the response of Sus gene to abiotic stress remains largely unknown.the following results show that Sus gene play an important role in abiotic stress:1. we analyzed the expression pattern of GaSus3and GaSus4under salt-stress in cotton, found that theexpression pattern of the two genes shows consistent trends: the expression level increased in theroots, but declined in the leaf.2. Plant over-expression vectors p35S：： GaSus3and p35S：： GaSus4were constructed andtransformed into the Arabidopsis separately. Transgenic plants were obtained successfully by floraldip method.3. We found that plants with over expressing GaSus3developed ectopic trichomes on the stem andfloral organs. This confirmed GaSus3gene play a role in the fiber development from the side.4. Compared to the wide type plants, the salt resistance ability of GaSus3transgenic plants wereenhanced significantly under NaCl treatments. For example, under salt-stress concentration, thegrowth rate of the wild-type Arabidopsis is severely suppressed, while the transgenic Arabidopsisshow less impact. Transgenic plants show better seed germination rate and relatively longer rootlength than wild type, which can support plant growth under stress condition. Besides, the leaf oftransgenic plants are less harmed which keep more green blade, whereas the wild-type Arabidopsisshows early yellowing and death.5. A relative significantly higher Catalase activity has been found in transgenic plant compared withthe wide type before and after salt stress treatment, which indicate that over expression of GaSus3can significantly enhance the plants ROS scavenge ability.All these results help to establish the basis of study the functions of Sus genes in response of cottonto salt stress in the future. |
PDF Full Text Request