The Study Of Microorganisms In Qinghai-Tibet Plateau Permafrost Regions And Identification Of A New Species D40P~T

Qinghai-Tibet Plateau permafrost regions as a typical representative of the global permafrost environment, in an increasingly serious impact on the greenhouse effect, the area is diminishing. Although China is the world’s third big country permafrost, frozen soil science research in a leading position in the world, but for permafrost microorganisms still poorly understood. All this indicates that further analysis of the Qinghai-Tibet Plateau permafrost microorganisms in the field of microorganisms is an urgent research.Six soil samples of article were taken from the Qinghai-Tibet Plateau permafrost. The samples were pretreatment, were extracted genomic using soil DNA extraction kit and were high-throughput sequencing using Miseq method. Through several of analysis methods study the microbial community structure, such as species classification, OTU clustering, Alpha diversity and Beta diversity. Get the following results:The phyla Proteobacteria, Armatimonadetes, Chloroflexi. Fibrobacteres, Therm, Deinococcus-Tmus, Chlorobi, Bacteroidetes, Gemm, Fusobacria, Cyanobacteria, Acidobacteria, Nitrospirae, Verrucomicrob, Firmicutes, Actinobacteria and unclassified Bacteria occurred in the samples, among which Proteobacteria and Firmicutes were dominant. The phyla Euryarchaeota, Crenarchaeota and unclassified archeae occurred in the samples, among which Crenarchaeota were dominant. The community structure of samples K25and K26only because of different sampling depthsand different, sampling area of K35may be the highest bacterial diversity.D40PT strains isolated from the K45soil samples, according to the manual identification of common bacterial systems to identify new species, measuring16S rDNA sequence comparison with its nearest phylogenetic species Sandarakinorhabdus limnophila, similarity of95.46%. Further were carried on a variety of indicators to identify experiment:microbial forms, physiological and biochemical characteristics, the cells chemical composition, G+C mol%and fatty acids, polar lipids, DNA-DNA hybridization etc. Determine the strain D40P1as new species of Sandarakinorhabdus.