Effects Of α-ketoglutarate On Growth In Ctenopharyngodon Idellus

The aim of this experiment was to investigate the effects of a-Ketoglutarate on growth performance, immune and intestinal mucosa of grass carp.Test one:The grass carp with an average body weight of35.64±1.39g were randomly allocated into5groups with3replicates of30fish. The experiment made cages(1.5*1.5*1.5) in the pond.The control group was fed with the basal diet,whilt trial groups were fed with the diet added with2.5g/kg,5g/kg,7.5g/kg, lOg/kg AKG. The experiment lasted for60days. The results show that:Adding different concentration of AKG on grass carp feed, the survival rate had no significant effect (P>0.05). When adding concentration is7.5g/kg, the rate of weight gain and specific growth rate of grass carp、the ratio of RNA/DNA in muscle、the sod of the blood、the lysozyme of the blood、the GDH and y-GT in the liver、the muscular thickness of foregut and midgut are significantly increased (P<0.05); and the MDA in the bloodn the urea content in the blood、the liver TP、the villus width of for-egut are significantly decreased (P<0.05); while there was no significant change on the muscle protein, fat, and amino acids and the TP、ALP and ALB in the blood and the villus width and the number of goblet cells of midgut (P>0.05); the GLU content present a tendency of increase with the increase of concentration of added and the ma-ximum is the10g/kg。Based on the analysis discussion, AKG opti-mum adding amount was7.5g/kg.Test two:In AKG the optimum adding amount was7.5g/kg, th-e grass carp with an average body weight of10.81±0.68g were ran-domly allocated into2groups with4replicates of30fish. The ex-periment made cages(1.5*1.5*1.5) in the spawning pool.The control group was fed with the basal diet,whilt trial groups were fed with the diet added with7.5g/kg AKG. The experiment lasted for60d-ays. Results indicate that:AKG can significantly increase the GS, GSH, GSH-Px, CAT, SOD, T-AOC, and HK activity in plasma (P<0.05), and significantly dincrease MDA, Hb, no C3in plasma (P<0.05), and no significant influence on GST, MetHb, ADA, LSZ, ACP(P>0.05). the muscle glycogen and ADA in muscle decreased significantly (P<0.05), but RNA/DNA ratio and total protein content significantly increased(P<0.05); AKG can significantly improve the liver GSH and GS, while significantly reduce the content of TP and liver glycogen in liver (P<0.05); AKG can significantly increase the content of the intestinal protease and lipase (P<0.05), but there was no significant impact on the amylase content (P >0.05); and AKG can improve GSH of foregut and TP of midgut (P<0.05), at the same time reduce the intestinal ACP and NO content (P<0.05); the test two reconfirmed that the7.5g/kg AKG on growth performance, immune and intestinal mucosa of grass carp is of great importance...