Study On The Genetic Diversity Of3Natural Populations Of Macrobrachium Nipponense

11microsatellite locus were used to analyze the genetic diversity of87individuals of in three natural populations of Macrobrachium nipponense Dongting, Poyang and Longgan. The genetic variation within population and the genetic relationship among populations was also analyazed. It can provide the basis of science conservation and reasonable utilization of indigennous Macrobrachium nipponense resource.The47microsatellite loci were selected from GeneBank and the published macrosatellite primers. The results showed that11microsatellite locus were highly polymorphic. A total of90alleles were detected, among which69polymorphic.,each loci had8.182alleles. In these natural populations the average number of allele (A) ranged from32to38, and the value of average polymorphism information content (PIC) was0.5907to0.6430, the value of average expected heterozygosity (He) was0.6577to0.7055, the value of average observed heterozygosity (Ho) in three populations ranged from0.5141to0.5799. Shannon index were greater than1. Fis were positive, average inbreeding coefficient of populations was19%. FST Value showed that the whole populational differentiation degree was lower. Most variation occurred within populations (94.62) among population (5.38). The results showed that the genetic diversity is richer in three natural populations of Macrobrachium nipponense, and Poyang is the most abundant genetic diversity population and is the lowest level of genetic differentiation. The average genetic distance was0.1888. Results of the clustering analysis showed that the population collected from Longgan Lake and from Poyang Lake were closest in relationship, while the population collected from Longgan Lake and from Dongting Lake were farthest in relationship.