The Research About The Variation Regulation Of Soil Chemical Properties And Microorganisms In The Process Of Reclaimed Water Reuse

Reclaimed water is the regeneration of urban sewage treatment system through filtration, precipitation and coagulation, meeting a specific water quality standard, which has been widely used in agricultural irrigation, industrial production, city miscellaneous, groundwater recharge and many other aspects. Reclaimed water reuse is the experimental group, tap water irrigation is the control group. We adopt indoor simulation method, research the impact of reclaimed water reuse on soil chemical properties, soil microbial amount and microbial community structure, explore the relationship between soil chemical properties and microbial amount, clear corresponding relationship between soil chemical properties and microbial community structure. In order to provide theoretical support for assessing reclaimed water reuse safety and improving reclaimed water treatment technology.Test and analysis soil chemical properties of reclaimed water and tap water irrigation area(organic matter, total nitrogen, total phosphorus, available phosphorus, pH value, soil water content), clearing soil chemical properties change rule in the process of reclaimed water reuse. The results showed that soil organic material and total nitrogen content showed a trend of increasing with reclaimed water reuse, speculated that reclaimed water reuse can increase soil nutrients and improve soil fertility to a certain extent. Reclaimed water had no significant effect on soil total phosphorus and available phosphorus, within the scope of the soil element security content. Reclaimed water had no significant effect on soil water content and pH, it didn’t cause acidification and alkalinization.According to traditional method of microbial isolation and culture to research the impact of reclaimed water reuse on soil microbial quantity and community structure change rule. The results showed reclaimed water reuse can improved bacteria and actinomyces quantity in 0~20 cm layer soil, but it had little effect on bacteria, actinomyces and fungi amount of 20~40 cm and 40~60 cm layer, the microbial number of each soil sample was 0~20 cm>20~40 cm>40~60 cm, and difference was significant(P<0.05). Culturable bacteria isolated from reclaimed water reuse area is distributed in 15 genera, Acinetobacter sp. is the dominant fungi, Aerococcus sp., Halobacterium sp. and other six genera are endemic species. Culturable actinomyces isolated from reclaimed water reuse area is distributed in 9 genera, Streptomyces sp. is the dominant actinomyces genera, Actinoplanaceae sp. and Actinosynnema sp. are endemic species. Culturable fungi isolated from the reclaimed water reuse area is distributed in 12 genera, Penicillium sp. is the dominant fungi genera, Scopulariopsis sp., Rhipidium sp. and Cladosporium sp. are endemic species.Through SPSS 17.0(Statistical Product and Service Solutions) correlation analysis between soil chemical properties and microbial quantity showed the total number of bacteria, actinomyces, fungi were positively correlated with organic material, total nitrogen, total phosphorus and available phosphorus. Through CANOCO 4.5 redundancy analysis between soil chemical properties and microbial community structure showed soil microbial community structure presence a certain spatial difference with the change of chemical properties. Organic matter, total nitrogen, total phosphorus and available phosphorus have bigger impact on the microbial community structure than others physical-chemical factors, including organic matter, total nitrogen and available phosphorus have bigger impact on the distribution of Planococcus sp., Halobacterium sp., Actinosynnema sp., Actinoplanaceae sp.. Total phosphorus content has a bigger impact on the distribution of Neisseria sp..