Research On Preparation And Performance Of Oral Enteric Soluble Anti PRRSV-IgY Microcapsule

With the rapid development of modern biotechnology, protein and polypeptide drugs for new formulations has been widely used in people’s life, and play an invaluable role on the development of the pharmaceutical industry. However this kind of drugs is easily to be degraded by all kinds of digestive enzymes and it’s biological activity will weaken or lost when it reach the organism, So it was restricted by many factors of the oral medication aspect. The anti PRRSV-IgY microcapsules prepared by this experiment can solve this problem every well, the microcapsule can protect the anti PRRSV - IgY successfully through the gastric juice. At the same this will make anti PRRSV - IgY absorb and utilize drug to the utmost.So this late-model drugs has a broad scope in future.By studying the characteristics of three kinds of wall material of this experiments, the Xanthan gum with perfect performance as the first wall material embedding specific antibody, Sodium alginate as the second material and the first wall material bounded were fused to form composite wall material, preparation of microcapsules containing specific antibody, We developed a kind of microcapsule carrier of IgY, which can ensure the specific antibody was not the digestive juices in the stomach damage, but also can ensure the sustained release microcapsules in the intestine. The results were as follows:1:Different wall materials to model drug bovine serum albumin (BSA) adsorption effect: Through the research of three kinds of carrier materials. draw the conclusion: Xanthan gum concentration was 0.05% (W/V%), adsorption of a model drug BSA can best: 86.34%.2:Determine the most suitable concentration of the first wall material of microcapsules:Based on the determination of encapsulation, drug loading effect was found in 0.05%(W/V%)Xanthan as the first wall material for microcapsules prepared, the encapsulation efficiency and drug loading is better than the other combinations, the encapsulation efficiency and drug loading respectively: 89.96% and 19.77%.3:Optimization and Performance microcapsules preparation process: The microcapsules formed the entrapment efficiency and drug loading can reach 91.12% and 20.75%.the total amount of released drug close to 85%.4:Determination of load performance IgY microcapsules (anti PRRSV-IgY):Under this process microcapsules prepared anti PRRSV-IgY loss rate was 6.65%, encapsulation efficiency of microcapsules was 86.83%, the drug loading was 18.89%. The release amount of 2h in artificial gastric juice in anti PRRSV-IgY was 8.79%; transferred to the artificial intestinal fluid, the cumulative release amount of 4h in the simulated intestinal fluid was 70.77%, persistent after 6h, the cumulative release amount of drug can reach 82.77%. At the same time, anti PRRSV-IgY microcapsules prepared for strong resistance to temperature, resistance to humidity and weak.