| [Objective] In the present study, learning and memory behaviors in male mice exposed to single Bisphenol A(BPA), single lead (Pb) or combined were assessed in order to explore the nervous toxicology of BPA and Pb, and further provide the theoretical basis.[Method] 60 mature healthy Kunming mice were obtained from the Experimental Animal Center of Shanxi Medical University. After acclimatization for 1 week, one male and two females were placed in a cage together for mating. As soon as the vaginal plug was established, the females were separated from male mice, and were randomly divided into control and experimental groups as follows:(1) control group; (2) BPA group:200mg/kg/d; (3) lead group:300mg/1 Pb (CH3COO) 2; (4) BPA plus lead group:(200mg/kg/d) and Pb (300mg/1 Pb (CH3COO) 2. Using Open-field test and Object recognition test, the behavior of the male mice at 4 weeks of age were observed. After the behavioral tests, the mice were killed and the brain was removed. Appling diverse methods as Real-time PCR and Western blotting, this study observed the changes of hippocampus glutamate receptors (GluR1 and NMDAR1), in order to explore the mechanism of effects of BPA and lead exposure on learning and memory during the postnatal developing stage for scientific diagnosis and target drug design.[Result] 1. The results showed that BPA and lead group treatments significantly decreased the body weight of male at 4 weeks,comparing with the control group.2. Open field test results showed that the exploratory behavior of filial mice was inhibited by BPA and lead. OR results showed that the BPA and lead significantly rescued learning and memory.3. Embryonic BPA exposure decreased the mRNA and protein expressions of GluR1 and NMDAR1 in the hippocampus of male offspring.[Conclusion] The present results revealed that embryonic BPA or/and lead exposure has a side effect on learning and memory in male during the postnatal developing stage. It suggests that the mechanism of BPA and lead on brain damage may involve in changes of mRNA and protein expressions in the hippocampus glutamate receptors (GluR1 and NMDAR1). |
